ZDF Rat Diabetic Ovarian Fibroblasts

Catalog No. RD-6072

Suggested Medium: Fibroblast Medium /w Kit – 500 ml

Catalog No. M2267

Product Description

ZDF Rat Diabetic Ovarian Fibroblasts from Cell Biologics are isolated from ovarian tissue of male, 9 week old laboratory ZDF rat. Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics Culture Complete Growth Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10⁶ cells per ml and is delivered frozen.

Product Testing

ZDF Rat Diabetic Ovarian Fibroblasts from Cell Biologics are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Laboratory Applications

ZDF Rat Diabetic Ovarian Fibroblasts can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage of Cell Biologics Products

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.

Authorized Uses of Cell Biologics Products

ZDF Rat Diabetic Ovarian Fibroblasts from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics, without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution, for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

Cell Biologics公司提供高质量的基因改造小鼠及正常小鼠原代内皮细胞，小鼠干细胞，小鼠原代上皮细胞，小鼠骨髓细胞，小鼠胚胎细胞，小鼠白细胞和其它小鼠细胞。为科研者的研究提供高质量的原代细胞，并针对实验中的问题提供更多有效的和低成本的方法。 Cell Biologics细胞生物制剂公司总部位于美国芝加哥，是全球独家提供Jackson Lab基因敲除小鼠原代细胞的专业公司，产品已获美国农业部SPF认证，并经中国进出口商品检验检疫局检验准许进口。   自成立以来，Cell Biologics已开发出13个品系,1000多种可直接使用的Jackson Lab基因敲除小鼠、正常野生型小鼠、大鼠原代细胞，必将填补细胞生物学 界研究的空白。另外，Cell Biologics拥有AAALAC认证的动物实验室，提供Jackson Lab与国外其他著名实验室所有允许商用的基因敲除小鼠原代细胞分离服务.   此次美国之行第三站，就是位于美国芝加哥的Cell Biologics细胞生物制剂公司。Cell Biologics的VP Wendy Gao女士和Asrar B. Malik博士与妙通生物CEO张金保先生进行了深度的会谈，会后也一起参观了Cell biologics的研发实验室。 图为妙通生物CEO张金保先生（右一）Asrar Malik博士（居中）和Wendy Gao（左一）于办公室的合影：