Cell Biologics/Mouse Primary Diabetic Liver Sinusoidal Endothelial Cells/MD-6017/

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¥12400.00
货号:MD-6017
浏览量:127
品牌:Cell Biologics
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商品描述

Mouse Primary Diabetic Liver Sinusoidal Endothelial Cells

Catalog No.  MD-6017

Suggested Medium: Endothelial Cell Medium /w Kit – 500 ml

Catalog No.  M1168

Product Description

Mouse Primary Diabetic Liver Sinusoidal Endothelial Cells from Cell Biologics are isolated from the liver of Diabetic (db/db) mice. Mouse Primary Diabetic Liver Sinusoidal Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.

Product Testing

Mouse Primary Diabetic Liver Sinusoidal Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R&D System) or CD31/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Laboratory Applications

Mouse Primary Diabetic Liver Sinusoidal Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage of Cell Biologics’ Products

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.

Authorized Uses of Cell Biologics’ Products

Mouse Primary Diabetic Liver Sinusoidal Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Appropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media (catalog number M1168) and Coating Solution for appropriate endothelial cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

Cell Biologics公司提供高质量的基因改造小鼠及正常小鼠原代内皮细胞,小鼠干细胞,小鼠原代上皮细胞,小鼠骨髓细胞,小鼠胚胎细胞,小鼠白细胞和其它小鼠细胞。为科研者的研究提供高质量的原代细胞,并针对实验中的问题提供更多有效的和低成本的方法。 Cell Biologics细胞生物制剂公司总部位于美国芝加哥,是全球独家提供Jackson Lab基因敲除小鼠原代细胞的专业公司,产品已获美国农业部SPF认证,并经中国进出口商品检验检疫局检验准许进口。   自成立以来,Cell Biologics已开发出13个品系,1000多种可直接使用的Jackson Lab基因敲除小鼠、正常野生型小鼠、大鼠原代细胞,必将填补细胞生物学 界研究的空白。另外,Cell Biologics拥有AAALAC认证的动物实验室,提供Jackson Lab与国外其他著名实验室所有允许商用的基因敲除小鼠原代细胞分离服务.   此次美国之行第三站,就是位于美国芝加哥的Cell Biologics细胞生物制剂公司。Cell Biologics的VP Wendy Gao女士和Asrar B. Malik博士与妙通生物CEO张金保先生进行了深度的会谈,会后也一起参观了Cell biologics的研发实验室。 图为妙通生物CEO张金保先生(右一)Asrar Malik博士(居中)和Wendy Gao(左一)于办公室的合影: