Description	Black-Gold®IIisanovelhaloaurophosphatecomplexwhichlocalisesmyelinwithinthecentralnervoussystem.TheBlackGoldIIReady-to-Dilute(RTD™)StainingKitallowsyoutolocalisemyelin,bothindividualfibresandtracts,alongwiththeoptionofco-localisingcellbodiesviatheToluidineBluecounterstain.BlackGoldIIlabelledmyelinatedfibresappearnearlyblackwhiletheToluidineBlueOlabelledcellularNisslbodiesareblueunderbrightfieldIllumination. BlackGoldIIcandemonstrateandcharacterisespecificmyelinchangesassociatedwithexposuretodiverseneurotoxicantsincludingkainicacid,domoicacid,3-nitropropionicacid,Fluoro-Goldandisoniazid.BlackGoldIIcanalsobecombinedwithotherhistochemicalMarkersincludingNisslstains,retrogradelytransportedfluorescenttracersandfluorescentmarkersofneuronaldegeneration.TheadvantagesassociatedwiththeBlack-GoldIIincludehighresolution,highcontrast,shorthistochemicalprocessingtime,versatilityandconsistentreproducibility.
OtherNames	blackandgold
Applications	BlackGoldIIisahighresolutionmyelinstainwithamyloidplaquecounterstain.Itsuseistailoredtostudiesusingformalinorparaformaldehydefixed,non-paraffinembedded,non-solventprocessedbraintissue.Itcanbeusedwithboththickandthinsections.Forthicksections,gelatincoatedslidesorslidesspeciallydesignedtobindtissuessectionsshouldbeusetoavoidsectionloss.Free-floatingsectionscanbeusedaswellbutsectionsareeasiertohandleandtransferwhenmountedonslides.Asuggestedmethodforthicksectionsisprovidedasaguide:Eitherfrozenorvibratomesectionsarecutatathicknessof20-50umandcollectedin0.1Mneutralphosphatebuffer.Thesectionsarethentypicallymountedon1%gel-coatedslidesandthenairdriedonaslidewarmer(at50degreesC)foratleastanhouruntilthroughlydriedandadheredtotheslide.Thesectionscanbestainedloose,althoughthesectionsareeasiertohandlewhenmountedonslides.Themountedsectionswererehydratedindistilledwaterfor2minutesbeforebeingprocessedinthestainingsolutions.
Form	ThereagentsintheBlackGoldkitareallsuppliedinaliquidformatandareready-to-dilute.
Storage	Thekitcanbetransportedatroomtemperature.Oncereceived,thekitcanbestoredforupto12monthsat4°Cprotectedfromlight.Dilutedsolutionscanbestoreduptoonemonthat4°Cprotectedfromlight.
SpecificReferences	YingYLetal.(2014)Adultneuralprecursorcellsfromthesubventricularzonecontributesignificantlytooligodendrocyteregenerationandremyelination.JNeurosci.2014Oct15;34(42):14128-46
KitComponents	Black-GoldII(Dilute1:10priortouse)-10mLSodiumThiosulfate,fixative(Dilute1:10priortouse)-10mLToluidineBlueO(Dilute1:10priortouse)-10mLAceticAcid(Dilute1:10priortouse)-10mL
ReagentKitprotocol	TR-100-BG_April2012.pdf
MSDS	MSDS_TR-100-BG_BGT_as_at_Apr2014.pdf

Biosensis是一家由一群生命科学家和商界人士组成的公司，致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有100多年的经验，在生命科学研究和诊断市场的生命科学抗体、试剂和试剂盒的商业化方面拥有50多年的经验。因此，我们非常理解研究人员需要高质量的有效试剂。 Biosensis专注于神经科学的抗体和试剂，特别强调神经营养素和神经营养素受体，近30年来，Biosensis一直是这两个领域的全球领先者和OEM供应商。除了神经营养素，我们的神经科学产品组合还包括研究神经退行性疾病、神经发育和神经新陈代谢的产品。重点领域包括阿尔茨海默氏症、帕金森氏症和肌萎缩侧索硬化症，以及自噬、代谢和应激障碍，包括代谢综合征研究、肥胖、神经免疫学和炎症。 Biosensis的产品范围不仅包括不断增长的神经科学研究抗体系列，还包括广泛和不断扩大的200+定量研究ELISA系列、组织染色和细胞可视化试剂，以及针对神经科学和细胞疾病研究的纯化蛋白。我们的抗体和试剂已被广泛应用于多种技术，包括蛋白质印迹、免疫组织化学、流式细胞仪分析、共聚焦显微镜实时成像、生物抑制以及细胞培养和克隆。最后，Biosensis还拥有340多份同行评审的研究出版物以及精湛的技术支持和经验，能够为神经科学及其相关研究领域提供业界无与伦比的客户支持和科学专业知识。