DESCRIPTION:

Afullyvalidated,highlyspecificaffinitypurifiedrabbitantibodytothreonine-17phosphorylatedformsofphospholamban

Phospholamban(PLB/PLN)isasmalltransmembraneproteinwhichplaysanimportantroleincontrollingtheactivityofthesarcoplasmicreticulumATPase(SERCA2a)ofcardiacmuscleduringcalciumsequestration.

PhospholambanisphosphorylatedonseparateaminoacidresiduesbycAMP-dependent,andcGMP-dependent(serine-16) andCa²⁺/CaM-dependent(threonine-17)proteinkinasesinresponsetoβ-adrenergicstimulation.AkthasalsobeenshowntophosphorylateThr-17.Theresultisanincreasedcalciumpumpactivitywhichreducesthetimecourseofthecalciumtransient,increasesthecalciumloadinthesarcoplasmicreticulum,andconsequently,producesalargercalciumtransientatthenextactionpotential.However,alterationinthishomeostaticinteractionhasbeenshowntoresultinheartfailure.

Testedapplications:Westernblot(1:5000dilution).SuppliedaslyophilisedaffinitypurifiedIgG.

DATASHEET:

Description	AffinitypurifiedRabbitpolyclonalantibody(A010-13AP)tophospholambanphosphoThr-17
Immunogen	Syntheticpeptide(R9SAIRRAST(PO3H2)IEY20)correspondingtoaminoacidssurroundingthephosphorylatedthreonineresidueatposition17ofphospholamban,whichwasconjugatedtokeyholelimpethemocyanin(KLH)
Isotype	IgG
Purification	ProteinGaffinitypurified.ElutedincitratebufferpH2.8,neutralisedwithTris-base.StABIliseraddedto20%v/v
Speciesreactivity	Theantibodyrecognisesmonoandoligomericphospholambanwhenphosphorylatedatthreonine-17.BindingoftheantibodytoitstargetepitopeisblockedinthepresenceofaphosphopeptidecontainingthePLBPhosphoThr-17epitope.
Testedapplications	WB
Recommendeddilution	WB1:1000
Optimization	Optimaldilutionstobedeterminedbyenduser
Storage	Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin100µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze–thawcycles.
Storage	Lyophilised:4°CReconstituted:-80°C
Datasheet	A010-13AP
MSDS	A010-13AP
Regulatorystatement	Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse.

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