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蚂蚁淘在线 / 品牌中心 / Alomone / 阿洛蒙/抗GPR65（TDAG8）（细胞外）-FITC抗体/AGR-043-F/10 x 50µl

阿洛蒙/抗GPR65（TDAG8）（细胞外）-FITC抗体/AGR-043-F/10 x 50µl

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￥10540.00

货号：AGR-043-F-50µl

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商品描述

Cat#:AGR-043-F

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:h,m,r

Immunogen

Peptide(C)DYTWNKDNWTFSP,correspondingtoaminoacidresidues72-84ofhumanGPR65(AccessionQ8IYL9).Extracellular,1^stloop.

Anti-GPR65 (TDAG8) (extracellular)-FITC Antibody -

Accession(Uniprot)NumberQ8IYL9

GeneID8477

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyMouse–12/13aminoacidresiduesidentical;rat-11/13aminoacidresiduesidentical.

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

LabelFluoresceinisothiocyanate(FITC).

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution50µldoubledistilledwater(DDW).

Antibodyconcentrationafterreconstitution1mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl1µgpeptideper1µgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AGR-043),anddirectflowcytometry(labeledantibody).

Applications:fc

Mayalsoworkin:ic,lci

Directflowcytometry

DirectflowcytometryofliveintacthumanJurkatT-cellleukemiacells:

^___Cells.
^___ Cells+rabbitIgGisotypecontrol-FITC.
^___Cells+Anti-GPR65(TDAG8)(extracellular)-FITCAntibody (#AGR-043-F),(5µg).

DirectflowcytometryofliveintactmouseM1myeloidleukemiacells:

^___Cells.
^___ Cells+rabbitIgGisotypecontrol-FITC.
^___Cells+Anti-GPR65(TDAG8)(extracellular)-FITCAntibody(#AGR-043-F),(5µg).

References

Justus,C.R.etal.(2013)Front.Physiol.4,354.
Justus,C.R.etal.(2017)J.Transl.Med.15,204.

Scientificbackground

GPR65,alsocalledTDAG8(T-celldeathassociatedgene8protein),isapH-sensingG-proteincoupledreceptor(GPCR)activatedbyacidicextracellularpHthroughtheprotonationofseveralhistidineresiduesinthereceptor’ssequence.GPR65isinvolvedincancercellmetastasisandproliferation,immunecellfunction,inflammation,andbloodvesselformation¹.

HumanGPR65genehasbeenmappedtoalocationthatisassociatedwithTcelllymphomaandleukemiaabnormalities.

GPR65isprimarilyexpressedinimmunecellsandleukocyte-richtissuessuchascirculatingperipheralleukocytes,spleen,thymus,andtonsils.Expressionisalsodetected pain relevantlocisuchasthedorsalrootganglianeuronsandparticularlysmalldiameterneuronsresponsIBLefornociception^1,2.

GPR65hastheABIlitytoaffecttumordevelopmentandgrowth.OverexpressionofGPR65inLewislungcarcinomacellsincreasestumorgrowthinmiceandmayfacilitateresistancetoacidosis-mediatedcelldeath invitro throughproteinkinaseA(PKA)andERKrelatedpathways¹.

KnockdownofGPR65inNCI-H460humannon-smallcelllungcancercellsdecreasescellsurvivalinacidiccondition.AnotherstudyshowsthatactivationofGPR65byacidosiscanpromoteevasionofcellapoptosisunderglutaminestarvationanditsoverexpressionhasbeenreportedtotransformimmortalizedmammaryepithelialcells¹.

LyophilizedPowderControlAntigenIncluded

Lastupdate:20/03/2019

Anti-GPR65(TDAG8)(extracellular)Antibody(#AGR-043)isahighlyspecificantibodydirectedagainstanepitopeofthehumanprotein.Theantibodycanbeusedinwesternblot, immunohistochemistry,andlivecellflowcytometry.IthasbeendesignedtorecognizeGPR65fromrat,mouse,andhumansamples.

Anti-GPR65(TDAG8)(extracellular)-FITCAntibody(#AGR-043-F)isdirectlyconjugatedtofluoresceinisothiocyanate(FITC).Thislabeledantibodycanbeusedinimmunofluorescentapplicationssuchasdirectlivecellflowcytometry.

Forresearchpurposesonly,notforhumanuse

Directflowcytometry

DirectflowcytometryofliveintacthumanJurkatT-cellleukemiacells:

^___Cells.
^___ Cells+rabbitIgGisotypecontrol-FITC.
^___Cells+Anti-GPR65(TDAG8)(extracellular)-FITCAntibody (#AGR-043-F),(5µg).

DirectflowcytometryofliveintactmouseM1myeloidleukemiacells:

^___Cells.
^___ Cells+rabbitIgGisotypecontrol-FITC.
^___Cells+Anti-GPR65(TDAG8)(extracellular)-FITCAntibody(#AGR-043-F),(5µg).

References

Justus,C.R.etal.(2013)Front.Physiol.4,354.
Justus,C.R.etal.(2017)J.Transl.Med.15,204.

Alomone Labs是您离子通道研究的合作伙伴，是离子通道，GPCR和神经信号工具的领先供应商。我们专门生产基础科学研究中使用的一抗，小分子，蛋白质和肽。Alomone成立于1989年，现已成为全世界科学家使用的最全面的离子通道研究工具来源。Alomone的所有产品均经过生物测定验证，并以冻干粉形式提供，因此您可以对它们的生物活性和长保质期充满信心。立即索取信息 Alomone公司作为世界上***的离子通道专业供应商，它的离子通道抗体是世界上*全的、质量也非常好！目前被世界各地的科学家广泛的使用. Alomone Labs Alomone 公司是一家位于耶路撒冷的以色列著名的生物科技公司，专著于提供生命科学细胞生物学试剂和服务。Alomone 公司作为世界上最著名的离子通道专业供应商，它的离子通道抗体是世界上最全的、质量也非常好！目前被世界各地的科学家广泛的使用。试剂

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阿洛蒙/抗GPR65（TDAG8）（细胞外）-FITC抗体/AGR-043-F/10 x 50µl

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