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AlternativeName(s)ofTarget:5hmc;MABE176;5hmeC;5"hydroxymethyl;cytosine;5"OHmethylcytosine;5"OHmeC;5hmc;5-hmCImmunogen:5-HydroxymethylcytosineconjugatedtoBSA.
Specificity:Theanti-5-Hydroxymethylcytosineantibody,cloneAB3/63.3,detects5-hydroxymethylcytosine(hmc,5hmc),butnot5-methylcytosineorunmethylatedcytosine.
ApplicationNotes:Thisantibodycanbeusedtodetect5hmCbyimmunofluorescenceanddotblot.Zhangetal.(2014,PMID:24427299)usedtheoriginalratIgG2aformat(underthenameMABE176)inanimmunoprecipitationexperiment.
Antibodyfirstpublishedin:Ficzetal.Dynamicregulationof5-hydroxymethylcytosineinmouseEScellsandduringdifferentiation.Nature.2011May19;473(7347):398-402PMID:21460836Noteonpublication:Describesthegenerationanduseinimmunofluorescenceoftheoriginalantibody.
