AAT Bioquest/DiTO™-1 [equivalent to TOTO®-1] *5 mM DMSO Solution* *CAS 143413-84-7*/17575/0.2 ml

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¥56560.00
货号:17575
浏览量:127
品牌:AAT Bioquest
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Ex/Em(nm)514/535
MW1302.78
CAS#143413-84-7
SolventWater
StorageF/D/L
CategoryNucleicAcidDetection
DNADetection
RelatedLabelingCells
FluorescenceImaging
BiochemicalAssays
DiTO™-1ischemicallyequivalenttoTOTO®-1(TOTO®isthetrademarkofInvitrogen).DiTO™-1isacarbocyaninedimerwithgreenfluorescencesimilartoFITC.Itiscell-impermeantandeasilydistinguishedfromCy5andrhodaminesasanuclearcounterstainanddeadcellindicator.Itisnon-fluorescentintheabsenceofnucleicacids,andgeneratesaverybrightfluorescencesignaluponbindingtoDNA.DiTO™-1givesstrongandselectivenuclearstaininginculturedcellsandinparaffinsections.Simultaneouslabelingwithcell-permeableNuclearRed™LCS1dyeandcell-impermeantTOTO®-1canbeusedtoassesscellviABIlity.DiTO™-1andNuclearRed™bothhavemuchgreaterextinctioncoefficientsthanthatofDNA-boundpropidiumiodide.
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References&Citations
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1.   BonaccorsiS,GiansantiMG,CenciG,GattiM.(2012)ChromatinstainingofDrosophilatestes.ColdSpringHarbProtoc,2012.

2.   ShanL.(2004)Quinolinium,1,1"-[1,3-propanediylbis[(dimethyliminio)-3,1-propanediyl]]bis[4-[3-(3-methyl-2(3H)-benzothiazolylidene)-1-propen-1-yl]-,iodide(1:4).In:MolecularImagingandContrastAgentDatabase(MICAD),Bethesda(MD).

3.   HenNEBergerR,BirchD,BergquistP,WalterM,AnitoriRP.(2011)ThefluorescentdyesTO-PRO-3andTOTO-3iodideallowdetectionofmicrobialcellsinsoilsampleswithoutinterferencefrombackgroundfluorescence.Biotechniques,51,190.

4.   ChoH,IndigGL,WeichertJ,ShinHC,KwonGS.(2012)Invivocancerimagingbypoly(ethyleneglycol)-b-poly(varepsilon-caprolactone)micellescontaininganear-infraredprobe.Nanomedicine,8,228.

5.   HaberlS,MiklavcicD,PavlinM.(2010)EffectofMgionsonefficiencyofgeneelectrotransferandoncellelectropermeabilization.BioelectRochemistry,79,265.

6.   GlaserK,WilkeK,WepfR,BielSS.(2008)Visualizingnucleiinskincryosections:viableoptionsto4"6-diamidino-2-phenylindolforconfocallasermicroscopy.SkinResTechnol,14,324.

7.   PheMH,DossotM,GuilloteauH,BlockJC.(2007)HighlychlorinatedEscherichiacolicannotbestainedbypropidiumiodide.CanJMicrobiol,53,664.

8.   MartinRM,LeonhardtH,CardosoMC.(2005)DNAlabelinginlivingcells.CytometryA,67,45.

9.   PheMH,DossotM,BlockJC.(2004)Chlorinationeffectonthefluorescenceofnucleicacidstainingdyes.WaterRes,38,3729.

10.   LuppensSB,BarbarasB,BreeuwerP,RomboutsFM,AbeeT.(2003)SelectionoffluorescentprobesforflowcytometricviabilityassessmentofListeriamonocytogenesexposedtomembrane-activeandoxidizingdisinfectants.JFoodProt,66,1393.


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AAT Bioquest AAT Bioquest是一家位于美国的生物公司,前身为ABD Bioquest,总部位于加利福尼亚州。专门从事光学检测技术十多年,一直致力于光谱学检测领域技术的创新和突破。其独特的光学检测技术,综合了化学、生物学和信息学等各个领域的研究,引领了比色、荧光和发光技术新一代光学探针的浪潮。AAT Bioquest在全球拥有强大的经验丰富的专业分销商网络,为从小型研究机构到《财富》500强企业的各类客户提供卓越的产品和定制服务。AAT Bioquest拥有超过5000项产品,包括Fluo-8®、Cal-520®、Cell Meter™、Amplite™、CytoTrace™和CytoTell™等产品线,这些品牌在生命科学研究领域获得了认可和好评。 AAT bioquest 提供酶、核酸、蛋白标记、小分子检测、细胞凋亡、钙离子、离子通道、膜电位检测等特色试剂盒及荧光探针。AAT Bioquest提供的客户定制服务包括比色、荧光和发光探针的合成,生化、细胞和诊断检测试剂的开发,以及使用AAT Bioquest已经验证的化合物库来筛选客户的目标化合物等。   l   酶的活性检测 l   核糖核酸检测 l   蛋白质化学 l   小分子检测 l   荧光标记探针 l   生物素及其衍生物 l   经典荧光标记染料 l   偶联剂 l   荧光淬灭剂 l   特优荧光标记染料   l   蛋白标记