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蚂蚁淘在线 / 品牌中心 / AAT Bioquest / AAT Bioquest/trFluor™ Eu goat anti-rabbit IgG (H+L)/16820/1 mg

AAT Bioquest/trFluor™ Eu goat anti-rabbit IgG (H+L)/16820/1 mg

价格
￥9900.00
货号：16820
浏览量：127
品牌：AAT Bioquest
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Overview
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Ex/Em(nm)346/617
MWN/A
CAS#N/A
SolventWater
StorageF/D/L
CategoryImmunology
FluorescentAnti-IgGs
RelatediFluor™DyesandKits
TagAntibodies
BiochemicalAssays
ManyBIOLOGicalcompoundspresentincells,serumorotherbiologicalfluidsarenaturallyfluorescent,andthustheuseofconventional,promptfluorophoresleadstoseriouslimitationsinassaysensitivityduetothehighbackgroundcausedbytheautofluorescenceofthebiologicalmoleculestobeassayed.Theuseoflong-livedfluorophorescombinedwithtime-resolveddetection(adelaybetweenexcitationandemissiondetection)minimizespromptfluorescenceinterferences.OurtrFluor™Euprobesenabletime-resolvedfluorometry(TRF)fortheassaysthatrequirehighsensitivity.trFluor™EuprobeshavelargeStokesshiftsandextremelylongemissionhalf-liveswhencomparedtomoretrADItionalfluorophoressuchasAlexaFluororcyaninedyes.ComparedtotheotherTRFcompounds,ourtrFluor™EuprobeshaverelativelyhighstABIlity,highemissionyieldandabilitytobelinkedtobiomolecules.ThistrFluor™Eugoatanti-rabbitIgG(H+L)conjugateiscommonlyusedasasecondstepreagentforindirectimmunofluorescentstaining,whenusedinconjunctionwithbiotinylatedprimaryantibodies.Itisaveryvaluabletoolforbiotin-streptavidin-basedbiologicalassaysandtestsusingTR-FRETplatform.
SpectrumAdvancedSpectrumViewer

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References&Citations
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1.   SavilleL,SpaisC,MasonJL,AlbomMS,MurthyS,MeyerSL,AtorMA,AngelesTS,HustenJ.(2012)Time-ResolvedFluorescenceResonanceEnergyTransferasaVersatileToolintheDevelopmentofHomogeneousCellularKinaseAssays.AssayDrugDevTechnol.

2.   LoMC,NgoR,DaiK,LiC,LiangL,LeeJ,EmkeyR,EksterowiczJ,VenturaM,YoungSW,XiaoSH.(2012)Developmentofatime-resolvedfluorescenceresonanceenergytransferassayforcyclin-dependentkinase4andidentificationofitsATP-noncompetitiveinhibitors.AnalBiochem,421,368.

3.   PailaYD,KombrabailM,KrishnamoorthyG,ChattopadhyayA.(2011)OligomerizationoftheSEROtonin(1A)receptorinlivecells:atime-resolvedfluorescenceanisotropyapproach.JPhysChemB,115,11439.

4.   MartikkalaE,Rozwandowicz-JansenA,HanninenP,Petaja-RepoU,HarmaH.(2011)Ahomogeneoussingle-labeltime-resolvedfluorescencecAMPassay.JBiomolScreen,16,356.

5.   GaboritN,LarbouretC,VallagheJ,PeyrussonF,Bascoul-MolleviC,CrapezE,AzriaD,ChardesT,PoulMA,MathisG,BazinH,PelegrinA.(2011)Time-resolvedfluorescenceresonanceenergytransfer(TR-FRET)toanalyzethedisruptionofEGFR/HER2dimers:anewmethodtoevaluatetheefficiencyoftargetedtherapyusingmonoclonalantibodies.JBiolChem,286,11337.

6.   LeyrisJP,RouxT,TrinquetE,VerdieP,FehrentzJA,OueslatiN,DouzonS,BourrierE,LamarqueL,GagneD,GalleyrandJC,M"KadmiC,MartinezJ,MaryS,BaneresJL,MarieJ.(2011)Homogeneoustime-resolvedfluorescence-basedassaytoscreenforligandstargetingthegrowthhormonesecretagoguereceptortype1a.AnalBiochem,408,253.

7.   PosokhovYO,KyrychenkoA,LadokhinAS.(2010)Steady-stateandtime-resolvedfluorescencequenchingwithtransitionmetalionsasshort-distanceprobesforproteinconformation.AnalBiochem,407,284.

8.   Alvarez-CurtoE,WardRJ,PedianiJD,MilliganG.(2010)LigandregulationofthequaternaryorganizationofcellsurfaceM3muscarinicacetylcholinereceptorsanalyzedbyfluorescenceresonanceenergytransfer(FRET)imagingandhomogeneoustime-resolvedFRET.JBiolChem,285,23318.

9.   KotaS,ScampaviaL,SpicerT,BeelerAB,TakahashiV,SnyderJK,PorcoJA,HodderP,StrosbergAD.(2010)Atime-resolvedfluorescence-resonanceenergytransferassayforidentifyinginhibitorsofhepatitisCviruscoredimerization.AssayDrugDevTechnol,8,96.

10.   VisserAJ,LaptenokSP,VisserNV,vanHoekA,BirchDJ,BrochonJC,BorstJW.(2010)Time-resolvedFRETfluorescencespectroscopyofvisiblefluorescentproteinpairs.EurBiophysJ,39,241.


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AAT Bioquest AAT Bioquest是一家位于美国的生物公司，前身为ABD Bioquest，总部位于加利福尼亚州。专门从事光学检测技术十多年，一直致力于光谱学检测领域技术的创新和突破。其独特的光学检测技术，综合了化学、生物学和信息学等各个领域的研究，引领了比色、荧光和发光技术新一代光学探针的浪潮。AAT Bioquest在全球拥有强大的经验丰富的专业分销商网络，为从小型研究机构到《财富》500强企业的各类客户提供卓越的产品和定制服务。AAT Bioquest拥有超过5000项产品，包括Fluo-8®、Cal-520®、Cell Meter™、Amplite™、CytoTrace™和CytoTell™等产品线，这些品牌在生命科学研究领域获得了认可和好评。 AAT bioquest 提供酶、核酸、蛋白标记、小分子检测、细胞凋亡、钙离子、离子通道、膜电位检测等特色试剂盒及荧光探针。AAT Bioquest提供的客户定制服务包括比色、荧光和发光探针的合成，生化、细胞和诊断检测试剂的开发，以及使用AAT Bioquest已经验证的化合物库来筛选客户的目标化合物等。   l   酶的活性检测 l   核糖核酸检测 l   蛋白质化学 l   小分子检测 l   荧光标记探针 l   生物素及其衍生物 l   经典荧光标记染料 l   偶联剂 l   荧光淬灭剂 l   特优荧光标记染料   l   蛋白标记