Species: Human Host Species: Sf9 insect cells MW: 124 kDa Genbank #: NM_001111307 Tag(s): N-terminal GST-tag a.a: 2–886(end) Description:Human PDE4A4B, also known as PDE4A isoform 1 (GenBank Accession No. NM_001111307), a.a. (2-end) with N-terminal GST-tag, MW= 124 kDa, expressed in a Baculovirus infected Sf9 cell expression system. UniProt P27815 Synonym(s): PDE4A isoform 1, PDE4A4B Specific Activity: ≥ 11,610 pmol/min/µg Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3, 5-cAMP to 5-AMP per minute at 37°C. Assay Conditions: Assayed using BPSs PDE4A Assay Kit, Cat. # 60340. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA,0.05% Tween-20, 200 µM cAMP, 2.5 kU 5-nucleotidase, andserial dilutions of PDE4A4B at 37°C for 20 min.Quantified by 5- nucleotidase cleaving the5 -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent. Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 4 mM glutathione,3 mM DTT and 20% glycerol Format: Aqueous buffer solution Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s): 1. Sullivan,M., et al., Journal Biochem. J. 333:693-703 (1998). 2. Huston E, et al., J Cell Sci. 2006 119:3799-810. (2006). Warning(s): Avoid freeze/thaw cycles. Scientific Category: PDE Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.

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