Species: Human Description:R-Phycoerythrin-labeled recombinant human Anti-TIGIT antibody recognizing the TIGIT binding region on immune cells. Assay Conditions: For flow cytometry, we recommend incubating cells on ice in dark for 15-30 minutes with 20 µg/ml of RPE anti-human TIGIT antibody. Formulation: 8 mM Na Phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol. Purification: Protein A affinity chromatography from HEK293 supernatants. Application(s): Useful for labeling cells expressing TIGIT for flow cytometry and immunofluorescence microscopy. Reference(s):

1. Yu, X., et al., Nat. Immunol. 2009; 10(1): 48-57.2. Stanietsky, N., et al., Proc. Natl. Acad. Sci. (USA). 2009; 106(42): 17858-17863.

Application Reference(s):1.CD155T/TIGIT Signaling Regulates CD8+ T-cell Metabolism and Promotes Tumor Progression in Human Gastric Cancer(2017)

Warning(s): Avoid freeze/thaw cycles. Store diluted antibody with carrier protein (BSA 0.1 – 0.5%) at 4°C, stable for 2 weeks. Scientific Category: Immunotherapy Product Type: Antibody Data shown is lot-specific. Contact us for specific information on other lots.

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