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TheTDOFluorogenicInhibitorScreeningAssayKitisdesignedtomeasureenzymeinhibitionoftryptophan2,3-dioxygenase(TDO).TheTDOFluorogenicInhibitorScreeningAssayKitissimpletouseanddetectsfluorescenceatlongwavelengths,whichminimizespotentialerrorsduetocompoundinterference.Intheassay,theinhibitorandenzymeareaddedtoasamplecontainingL-Trpsubstrate.Aftera1hourincubationatroomtemperature,thefluorescencesolutionisaddedandincubatedat37°Cforfourhours.ActivityismeasuredbyreADIngsamplefluorescenceat&lamBDa;=510nmfollowingexcitationofthereactionproductatλ=400nm.
COMPONENTS:
Seegers,N.,etal.J.Biomol.Screen.2014.19(9):1266-74.


