Stemcell/Mouse TNF-alpha ELISA Kit/2 Plates/02030

价格
面议
货号:02030
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品牌:Stemcell
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商品描述

Overview

TheMouseTumorNecrosisFactor-alpha(TNF-α)ELISAKitisdesignedforthequantitativedetectionandmeasurementofmouseTNF-αinBIOLOGicalfluidssuchasserum,plasma,andcellculturesupernatants.TNF-αisprimarilyproducedbyactivatedTcellsandmacrophagesinresponsetoinflammationorinfection,butisalsoproducedbyBcells,NKcells,mastcells,neutrophils,dendriticcells,microglia,endothelialcells,smoothmusclecells,cardiomyocytes,andfibroblasts.TNF-αisapro-inflammatorycytokineandstimulatesanti-tumorresponsesinvitro.TheassayisbasedonthesandwichELISAmethod,inwhichsamplesareaddedtoELISAstripplatespre-coatedwithcaptureantibodiesspecificforthecytokine.Thecapturedcytokineisdetectedbyadditionofabiotinylateddetectionantibody,followedbystreptavidin-horserADIshperoxidase,whichbindsthebiotinylatedantibody.Additionofthechromogenicenzymesubstrate3,3’,5,5’tetramethylbenzidine(TMB)resultsinacoloredproductwithanintensitydirectlyproportionaltotheconcentrationofcytokineinthesample.Theconcentrationofthecytokineisdeterminedbycomparisontoaserialdilutionofthecytokinestandardanalyzedinparallel.
Components:
  • MouseTNF-alphaELISAKit,2plates(Catalog#02030)
    • MouseTNF-αELISAPlate,2x
    • MouseTNF-αStandard,1vial
    • MouseTNF-αBiotinylatedDetectionAntibody,30µL
    • SA-HRP,30µL
    • StandardReconstitutionBufferB,1mL
    • WashBuffer(20X),120mL
    • ELISADiluent,120mL
    • SA-HRPDiluent,25mL
    • TMBSubstrate,25mL
    • StopSolution,25mL
    • AdhesivePlateCovers,6x
  • MouseTNF-alphaELISAKit,10plates(Catalog#02031)
    • MouseTNF-αELISAPlate,10x
    • MouseTNF-αStandard,1vial
    • MouseTNF-αBiotinylatedDetectionAntibody,130µL
    • SA-HRP,130µL
    • StandardReconstitutionBufferB,1mL
    • WashBuffer(20X),5x120mL
    • ELISADiluent,3x120mL
    • SA-HRPDiluent,120mL
    • TMBSubstrate,120mL
    • StopSolution,120mL
    • AdhesivePlateCovers,30x
Subtype:
CompleteKits
CellType:
BCells;Cardiomyocytes,PSC-Derived;DendriticCells;EndothelialCells;GranulocytesandSubsets;Macrophages;MouseEmbryonicFibroblasts;MyogenicStemandProgenitorCells;NKCells;TCells
Species:
Mouse
Application:
ELISA
AreaofInterest:
Immunology;StemCellBiology
STEMCELL Technologies Inc.是一家以科技为导向的生物技术公司,主要为生命科学研究领域研发专业的干细胞培养基、细胞分选产品和其他辅助试剂,为全球70多个国家提供了超过1500种产品。