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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Overview
TheMouseTumorNecrosisFactor-alpha(TNF-α)ELISAKitisdesignedforthequantitativedetectionandmeasurementofmouseTNF-αinBIOLOGicalfluidssuchasserum,plasma,andcellculturesupernatants.TNF-αisprimarilyproducedbyactivatedTcellsandmacrophagesinresponsetoinflammationorinfection,butisalsoproducedbyBcells,NKcells,mastcells,neutrophils,dendriticcells,microglia,endothelialcells,smoothmusclecells,cardiomyocytes,andfibroblasts.TNF-αisapro-inflammatorycytokineandstimulatesanti-tumorresponsesinvitro.TheassayisbasedonthesandwichELISAmethod,inwhichsamplesareaddedtoELISAstripplatespre-coatedwithcaptureantibodiesspecificforthecytokine.Thecapturedcytokineisdetectedbyadditionofabiotinylateddetectionantibody,followedbystreptavidin-horserADIshperoxidase,whichbindsthebiotinylatedantibody.Additionofthechromogenicenzymesubstrate3,3’,5,5’tetramethylbenzidine(TMB)resultsinacoloredproductwithanintensitydirectlyproportionaltotheconcentrationofcytokineinthesample.Theconcentrationofthecytokineisdeterminedbycomparisontoaserialdilutionofthecytokinestandardanalyzedinparallel.
Components:
- MouseTNF-alphaELISAKit,2plates(Catalog#02030)
- MouseTNF-αELISAPlate,2x
- MouseTNF-αStandard,1vial
- MouseTNF-αBiotinylatedDetectionAntibody,30µL
- SA-HRP,30µL
- StandardReconstitutionBufferB,1mL
- WashBuffer(20X),120mL
- ELISADiluent,120mL
- SA-HRPDiluent,25mL
- TMBSubstrate,25mL
- StopSolution,25mL
- AdhesivePlateCovers,6x
- MouseTNF-alphaELISAKit,10plates(Catalog#02031)
- MouseTNF-αELISAPlate,10x
- MouseTNF-αStandard,1vial
- MouseTNF-αBiotinylatedDetectionAntibody,130µL
- SA-HRP,130µL
- StandardReconstitutionBufferB,1mL
- WashBuffer(20X),5x120mL
- ELISADiluent,3x120mL
- SA-HRPDiluent,120mL
- TMBSubstrate,120mL
- StopSolution,120mL
- AdhesivePlateCovers,30x
Subtype:
CompleteKits
CellType:
BCells;Cardiomyocytes,PSC-Derived;DendriticCells;EndothelialCells;GranulocytesandSubsets;Macrophages;MouseEmbryonicFibroblasts;MyogenicStemandProgenitorCells;NKCells;TCells
Species:
Mouse
Application:
ELISA
AreaofInterest:
Immunology;StemCellBiology


