Overview

Corning®Matrigel®hESC-QualifiedMatrix(CorningCatalog#354277)isasolublebasementmembraneextractoftheEngelbreth-Holm-Swarmtumor.Itgelsatroomtemperaturetoformagenuinereconstitutedbasementmembranerichinextracellularmatrixproteinssuchaslaminin,collagenIV,entactin,andheparansulfateproteoglycan(Bisselletal.;Kleinmanetal.1982&1986).Growthfactors,Collagenases,plasminogenactivators,andotherundefinedcomponentshavealsobeenidentifiedinthismatrix(McGuire&Seeds;Vukicevicetal.).Afterdilution,thematrixisusedtocoattissueculture-treatedculturewareforsupportingthefeeder-independentexpansionofhumanembryonicstem(ES)andinducedpluripotentstem(iPS)cells.Corning®Matrigel®hESC-QualifiedMatrixisanoptimizedsurfaceforstemcellresearchandhasbeenwidelyacceptedasanalternativesubstratetofeedercellsforthecultureofhumanESandiPScells(Drukkeretal.;Hammericketal.;Xuetal.2001&2004;Ludwigetal.2006a&2006b).IthasbeenqualifiedtobecompatIBLewithmTeSR™1(Catalog#85850),eliminatingtheneedfortime-consumingscreening,whileprovidingthereproducibilityandconsistencyessentialforhumanpluripotentstemcell(hPSC)research.Coupledwithfeeder-freehPSCmaintenancemediasuchasmTeSR™1,mTeSR™Plus(Catalog#05825),TeSR™-E8™(Catalog#05990),orTeSR™2(Catalog#05860),Corning®Matrigel®hESC-QualifiedMatrixcansuccessfullymaintainhumanESandiPScelllinesintheundifferentiatedstate.ThesecellsretaincharacteristichPSCmorphologyandexpressionofundifferentiatedcellMarkerssuchasOCT-3/4,SSEA-3,andTRA-1-60.Corning®Matrigel®hESC-QualifiedMatrixiscertifiedtobefreeoflactosedehydrogenaseelevatingvirus(LDEV/LDHV).Forthelot-specificdilutionfactor,refertotheMatrigel®CertificateofAnalysis,availableatwww.stemcell.com/coa.