PuromycinresistantGFP-HUVECswereselectedandshippedinproliferatingculturewith>90%confluence(cellsareprovidedatpassage3).ENDO-Growthmediumcontaining5%serumandgrowthsupplementisrecommendedforcellculture.Cellshaveanaveragepopulationdoublinglevelof>18whencultured.||Whenyoureceivethecells,leavetheflaskin37°CCO2incubatorfor1hour.Then,replacethetransportmediumwithfreshENDO-Growthmedium.Letthecellsgrowfor24hoursbeforesubculture.||Source:|GFPExpressingHumanUmbilicalVeinEndothelialCells,isolatedfromnormalhumanumbilicalveinsandtransfectedwithGFP-Lentiviralparticlesatpassage1.||CellNumber:|>90%confluent(>5x10e5cells)inT25flask||CellCharacterization:|CytoplasmicVWF/FactorVIII:>95%positivebyimmunofluorescence|CytoplasmicuptakeofDi-I-Ac-LDL:>95%positivebyimmunofluorescence|CytoplasmicPECAM1:>95%positivebyimmunofluorescence||GFP-HUVECsarenegativeforHIV-1,HBV,HCV,andmycoplasma.||ImportantNote:|CellsareprovidedeitherinaT-25flaskorasa1mlfrozenvial.||Store1mlvialsat-70°C.StoreT-25flasksat37°Cin5%CO2.Source:Normalhumanumbilicalveins|Purity:CellCultureGrade||ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBIOLOGical. USBio的生物化学品，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。USBio产品从经济实惠的研究数量到更大的批量可用于工艺开发和生产。我们的客户包括位于美国和国外的大多数*的制药和生物技术公司。