Description:
ELISAKitFeatures&Benefits:
ForResearchUseOnly.Notforuseindiagnosticprocedures.
TheMicroVueHelicalPeptideisatesttomeasuretypeIcollagenhelicalpeptideα1(I)620-633inurine,aMarkerforresearchingcollagendegradation.
TypeIcollagenisdegradedduringboneresorption,releasingintocirculationpeptidesofvariousmolecularweightsthatarefurtherdegradedand/orreleasedintourine.Apeptideconsistingofresidues620-633derivedfromthehelicalregionoftheα1chainwasisolatedfromtheurineofapatientwithPaget"sdisease.
| Feature | Benefit |
|---|
ProductSpecifications:
| ProductSpecName | ProductSpecData |
|---|---|
Format | ELISA |
Test/kit | 96wells/plate |
Sampletype | Urine |
PerformanceCharacteristics
Method:Competitive
Analyte:HelicalPeptideα1(I)620-633
Specimen:Urine
SpecimenVolume:20µL
LOD*:8µg/L
LLOQ*:20.2µg/L
HLOQ*:2514µg/L
PrecisionCVswithin-run:4%-8%
PrecisionCVsbetween-run:7%-17%
IncubationTime:19-25Hours
Cross-reactivity:Helicalpeptideα1(I)620-633100%IntactcollagenI,II,III,IV<1%Homologouspeptides(seePackageInsert)
*LOD=LimitofDetection,LLOQ=LowerLimitofQuantification,HLOQ=HigherLimitofQuantification
AbouttheAssay
Format
96-wellmicroplatewithreagentssufficienttotest40samplesinduplicate
AntibodySpecificity
Helicalpeptideα1(I)620-633;noreactivitywithintactcollagentypesI,II,III,IVormixturesofaminoacids;90%reactivitywithα1(III)homologouspeptide;noreactivitywithα1(I)orα2(II)homologouspeptides
SpeciesReactivity
Rat,Mouse,Rabbit,GuineaPig,Cat,Dog,Pig,Goat,Sheep,Cow,Horse,RhesusMacaque,CynomolgusMonkey,Baboon,Chimpanzee,HumanCellCulture
AssaySteps
Add20µLStandards,Controls,andsamples
Add150µLEnzymeConjugate
- Incubate18-24hoursat2°Cto8°C
- Wash3timeswith1xWashBuffer
- Add150µLSubstrate
- Incubate1houratroomtemperature
- Add100µLStopSolution
- Readabsorbanceat405nm
- DataReduction:4Parameter
- Correctforcreatinineconcentration
