Information Streptavidin, a bacterial protein isolated from Streptomyces avidinii, is similar to egg-white avidin in its ability to bind biotin, and has been used as a replacement for egg-white avidin because of its more favorable chemical properties.Conjugates of streptavidin are recommended for use with Biotin-SP-conjugated antibodies and Biotin-SP-conjugated ChromPure proteins.Usage Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:None(Warning:Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) Suggested Working Concentration or Dilution Range:ELISA:- 1-2 µg / mlWestern Blot:- 0.01-0.1 µg / mlHisto-/Cyto-Chemistry:- 1-2 µg / mlDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.Conjugate

Horseradish Peroxidase

Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

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This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

Jackson是全球专业从事生产和销售亲和纯化的二抗和纯化免疫球蛋白的公司，其产品主要销往到世界各地的大学科研所和医院里的动物研究以及生物医学研究机构的科学家。蚂蚁淘科技有限公司作为Jackson中国区代理，可以向中国客户提供Jackson的全系列产品。下图为Jackson（jacksonimmuno）公司授权蚂蚁淘科技有限公司（Ebiomall Scientific Inc.）作为Jackson在中国区域的代理授权书。