Information Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule sheep IgG.It also reacts with the light chains of other sheep immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with chicken, guinea pig, syrian hamster, horse, human, mouse, rabbit and rat serum proteins, but it may cross-react with immunoglobulins from other species.

F(ab")₂ fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab")₂ fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

Usage Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Store at 2-8°C – do not freeze. Prepare working dilution on day of use. Expiration date: six months from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity:The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads.Fc fragments and whole IgG molecules have been removed. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:0.05% Sodium Azide Suggested Working Concentration or Dilution Range:

1:50 - 1:200 for most applicationsDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Conjugate

Allophycocyanin (APC)

Amax: 650Emax: 660nm

Allophycocyanin (APC) is among several kinds of light-harvesting phycobiliproteins found in red, blue-green, and cryptomonad algae. APC is isolated from the blue-green alga Spirulina, and is chemically cross-linked for stability. After phycobiliproteins are conjugated to secondary antibodies, there is little fluorescence quenching, which results in conjugates of high specific fluorescence compared with conventional fluorophore-antibody conjugates. APC can be excited by light over a wide range of the visible spectrum, is highly water soluble, has a relatively low isoelectric point, and lacks potentially sticky carbohydrates.It should be noted that the relatively high molecular weight of APC may preclude its use in procedures requiring good penetration into cells and tissues. It is predominantly intended for surface labeling of cells for flow cytometry.

Images & References

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This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

Jackson是全球专业从事生产和销售亲和纯化的二抗和纯化免疫球蛋白的公司，其产品主要销往到世界各地的大学科研所和医院里的动物研究以及生物医学研究机构的科学家。蚂蚁淘科技有限公司作为Jackson中国区代理，可以向中国客户提供Jackson的全系列产品。下图为Jackson（jacksonimmuno）公司授权蚂蚁淘科技有限公司（Ebiomall Scientific Inc.）作为Jackson在中国区域的代理授权书。