Information Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule guinea pig IgG.It also reacts with the light chains of other guinea pig immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, syrian hamster, horse, human, mouse, rabbit,rat and sheep serum proteins, but it may cross-react with immunoglobulins from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Usage Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use.Extended Storage after Rehydration: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Borate - Sodium Phosphate, 0.15M NaCl, pH 8.5 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:0.05% Sodium Azide Suggested Working Concentration or Dilution Range:Histo-/Cyto-Chemistry:-1:20-1:200Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.Conjugate

4 nm Colloidal Gold

LM Grade colloidal gold reagents are intended for light microscopy and immunoblotting. Since signal intensity is relatively independent of particle size when silver enhancement is used, we offer 4 nm particles because this size may penetrate tissues better than larger particles. The 4 nm size may be used for electron microscopy in studies that require smaller particles since they are relatively uniform in size (coefficient of variation less than or equal to 15%), though small aggregates are not removed from this grade. Therefore, our 4 nm particles should only be used for electron microscopy with theunderstanding that the presence of small aggregates may give misleading results.

A detailed protocol for silver enhancement is provided with all orders for LM Grade products. All reagents involved in the light-insensitive silver enhancement reaction can be prepared easily in the laboratory. However, those who wish to use commercially available silver enhancement kits can continue to do so. All LM Grade colloidal gold-protein complexes are freeze-dried in buffer with stabilizers and a preservative. After reconstitution, they may be frozen in aliquots for extended storage.

Images & References

---

---

This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

Jackson是全球专业从事生产和销售亲和纯化的二抗和纯化免疫球蛋白的公司，其产品主要销往到世界各地的大学科研所和医院里的动物研究以及生物医学研究机构的科学家。蚂蚁淘科技有限公司作为Jackson中国区代理，可以向中国客户提供Jackson的全系列产品。下图为Jackson（jacksonimmuno）公司授权蚂蚁淘科技有限公司（Ebiomall Scientific Inc.）作为Jackson在中国区域的代理授权书。