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φ29 DNA Polymerase responsible for the replication of theBacillus Subtilis phage φ29 (1). The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand displacement activity (2) and a 3′→ 5′ proofreading exonuclease function (3).
Source of ProteinA recombinant E. coli strain carrying the φ29 DNA Polymerase gene from bacteriophage φ29.
Supplied in 10 mM Tris-HCl100 mM KCl0.1 mM EDTA1 mM DTT0.5% Tween-200.5% NP-4050% glycerolpH 7.4 @ 25°C
Supplied WithB7020 (10X φ29 DNA Polymerase Reaction Buffer)
10X φ29 DNA Polymerase Buffer (B7020)500 mM Tris-HCl100 mM (NH4)2SO440 mM DTT100 mM MgCl2pH 7.5 @ 25°C
Unit Definition1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dTTP into acid insoluble materialin 10 minutes at 30°C.
酶科技公司产品包括八类:超纯连接酶如T4连接酶、超纯poly酶、ArcherTM系列测序工具(Archer Products)、连接蛋白、DNA修饰酶、NGS文库制备(NGS Library Construction)、核酸和RNA酶。 Enzymatics is pleased to announce the launch of ZipScript™ One-Step RT-qPCR mix, a high efficiency RNA-dependent multiplex qPCR formulation. EnzScriptPhoenix Hot Start Taq产品列表: 品牌 名称 1 N2070-10-L 2 N2050-10-L 100 µmol Enzymatics 100 mM 75µmol dNTP Solution Set75 µmol Enzymatics 100 mM dATP Solution75 µmol Enzymatics 100µmol dNTP Solution Mix100 µmol Enzymatics 10x Uracil Cleavage System750 Reactions Enzymatics 2x HiFi PCR Master Mix1 rxn Enzymatics 5x ER/A-Tailing Enzyme Mix24 reactions Enzymatics 5X WGS Fragmentation Mix24 Reactions Enzymatics Bst X DNA Polymerase40,000 U Enzymatics DNA Polymerase I5,000 U Enzymatics E. coli DNA Ligase2,500 U Enzymatics E. coli fpg4,000 U Enzymatics E. coli Pyrophosphatase50 U Enzymatics E. coli Single-Stranded DNA Binding Protein1.5 mg