T3 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5′ phosphate and a 3′ hydroxyl termini in duplex DNA. The enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA. In the absence of 20-30% PEG 6000, T3 DNA Ligase displays a very low efficiency for blunt-ended ligation. (1) T3 DNA Ligase displays a higher efficiency for joining A/T overhangs than C/G matched ends. (1) T3 DNA Ligase retains 95% of its activity in 1.0 M NaCl or KCl, with an optimal concentration of 300 mM(1).

Source of ProteinA recombinant E. coli strain carrying the T3 DNA Ligase gene.

Supplied in20 mM Tris-HCl300 mM NaCl1 mM DTT0.1 mM EDTA50% glycerolpH 7.5 @ 25°C

Supplied With:B1010 (2X Rapid Ligation Buffer)

2X Rapid Ligation Buffer (B1010):132mM Tris-HCI20 mM MgCl₂2 mM DTT2 mM ATP15% PEG 6000pH 7.6 @ 25°C

Unit Definition1 unit is defined as the amount of T3 DNA Ligase required to ligate 50% of 100 ng DNA fragments with cohesive termini in 30 minutes at 23°C.

酶科技公司产品包括八类：超纯连接酶如T4连接酶、超纯poly酶、ArcherTM系列测序工具(Archer Products)、连接蛋白、DNA修饰酶、NGS文库制备(NGS Library Construction)、核酸和RNA酶。 Enzymatics is pleased to announce the launch of ZipScript™ One-Step RT-qPCR mix, a high efficiency RNA-dependent multiplex qPCR formulation. EnzScriptPhoenix Hot Start Taq产品列表： 品牌 名称 1 N2070-10-L   2 N2050-10-L 100 µmol Enzymatics 100 mM 75µmol dNTP Solution Set75 µmol Enzymatics 100 mM dATP Solution75 µmol Enzymatics 100µmol dNTP Solution Mix100 µmol Enzymatics 10x Uracil Cleavage System750 Reactions Enzymatics 2x HiFi PCR Master Mix1 rxn Enzymatics 5x ER/A-Tailing Enzyme Mix24 reactions Enzymatics 5X WGS Fragmentation Mix24 Reactions Enzymatics Bst X DNA Polymerase40,000 U Enzymatics DNA Polymerase I5,000 U Enzymatics E. coli DNA Ligase2,500 U Enzymatics E. coli fpg4,000 U Enzymatics E. coli Pyrophosphatase50 U Enzymatics E. coli Single-Stranded DNA Binding Protein1.5 mg