Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD⁺ as a cofactor.

Source of ProteinA recombinant E. coli strain carrying the cloned Taq DNA Ligase gene.

Supplied in10 mM Tris-HCl50 mM KCl1 mM DTT0.1 mM EDTA0.1% Tween-2050% glycerolpH 7.5 @ 25°C

Supplied With B6060 (10X Taq DNA Ligase Buffer)

10X Taq DNA Ligase Buffer (B6060)200 mM Tris-HCI250 mM KCl100 mM MgCl₂5 mM NAD0.1% Triton X-100pH 7.6 @ 25°C

Unit Definition1 unit is defined as the amount of Taq DNA Ligase required to join 50% of 1 µg of the 12-base cohesive ends of Lambda DNA cut with Sma I and Sal I in 50 µl 1X Taq DNA Ligase Buffer following a 10 minute incubation at 45°C.

酶科技公司产品包括八类：超纯连接酶如T4连接酶、超纯poly酶、ArcherTM系列测序工具(Archer Products)、连接蛋白、DNA修饰酶、NGS文库制备(NGS Library Construction)、核酸和RNA酶。 Enzymatics is pleased to announce the launch of ZipScript™ One-Step RT-qPCR mix, a high efficiency RNA-dependent multiplex qPCR formulation. EnzScriptPhoenix Hot Start Taq产品列表： 品牌 名称 1 N2070-10-L   2 N2050-10-L 100 µmol Enzymatics 100 mM 75µmol dNTP Solution Set75 µmol Enzymatics 100 mM dATP Solution75 µmol Enzymatics 100µmol dNTP Solution Mix100 µmol Enzymatics 10x Uracil Cleavage System750 Reactions Enzymatics 2x HiFi PCR Master Mix1 rxn Enzymatics 5x ER/A-Tailing Enzyme Mix24 reactions Enzymatics 5X WGS Fragmentation Mix24 Reactions Enzymatics Bst X DNA Polymerase40,000 U Enzymatics DNA Polymerase I5,000 U Enzymatics E. coli DNA Ligase2,500 U Enzymatics E. coli fpg4,000 U Enzymatics E. coli Pyrophosphatase50 U Enzymatics E. coli Single-Stranded DNA Binding Protein1.5 mg