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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Technical Documents
Description
Details
Dilution/Coupling Data
References
Technical Documents
Description
Sulfurizing Reagent II
Details
Usage
- Coupling: Sulfurizing: A 60 second sulfurization step time for DNA or a 360 second sulfurization step time for RNA is recommended.Sulfurization step must come before capping step to minimize phosphodiester formation.
- Deprotection: See TB_Sulfurizing II.pdf
| Specifications | |
|---|---|
| Diluent | 60% pyridine/40% Acetonitrile (fully dissolve in pyridine first, see notes below*) |
| Storage | Controlled room temperature or lower, dry |
| Stability | At least 6 months |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 40-4037-10 | 1 g | 1grams | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Expedite
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 40-4037-10 | 1 g | 1grams | 1 | 1 | 1 | 1 | 1 | 1 |
References
Literature Highlights
| Glen Report 18.1: Sulfurizing Reagent Ii - Stable In Solution And Optimized For Rna Sulfurization |
| Glen Report 18.1 Supplement: Sulfurizing Reagent Ii And Its Use In Synthesizing Oligonucleotide Phosphorothioates |
Related Products
Related Products
0.05M Sulfurizing Reagent II in Pyridine/AcetonitrileCatalog #: 40-4137
dA-ThiophosphoramiditeCatalog #: 10-1700
dC-ThiophosphoramiditeCatalog #: 10-1710
dG-ThiophosphoramiditeCatalog #: 10-1720
dT-ThiophosphoramiditeCatalog #: 10-1730
