Excelgen/FastÄQ™一步法SYBR绿色qRT PCR试剂盒,ROX&trade/EG-1024/10毫升

价格
¥30000.00
货号:EG-1024-10ml
浏览量:127
品牌:Excellgen
服务
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商品描述
Description

FastÄQTMOne-StepSYBRGreenqRT-PCRKit,ROX™isaconvenientandhighlysensitivesolutionforquantitativeRT-PCRofRNAtemplates(qRT-PCR)usingSYBR®GreenIdyedetectionandgene-specificprimersonallAppliedBiosystemsreal-timePCRsystemsexceptthe7500.CDNAsynthesisandPCRamplificationarecarriedoutinthesametubewithoutopeningbetweenprocedures.ThesystemhasbeenoptimizedtodelivermaximumRTPCRefficiency,sensitivity,andspecificity.TheproprietaryreactionbufferhasbeenspecificallyformulatedtomaximizeactivitiesofbothreversetranscriptaseandTaqDNApolymerasewhileminimizingthepotentialforprimer-dimerandothernon-specificPCRartifacts.ThekitiscompatIBLewithbothfastandstandardqPCRcyclingprotocols.HighlyspecificamplificationisessentialforsuccessfulqRT-PCRwithSYBR®GreenItechnology,sincethisdyebindstoanydsDNAgeneratedduringamplification.FastÄQTMTaqDNApolymerasecontainsmonoclonalantibodiesthatbindtothepolymeraseandkeepitinactivepriortotheinitialPCRdenaturationstep.Uponheatactivationat95ºC,theantibodiesdenatureirreversibly,releasingfullyactive,unmodifiedTaqDNApolymerase.

InstrumentCompatibility

  • AppliedBiosystems5700
  • AppliedBiosystems7000
  • AppliedBiosystems7300
  • AppliedBiosystems7700
  • AppliedBiosystems7900
  • AppliedBiosystems7900HT
  • AppliedBiosystems7900HTFast
  • AppliedBiosystemsStepOne™
  • AppliedBiosystemsStepOnePlus™
Features
  • HighsensitivityFastÄQTaqDNAPolymerase
  • Hot-starttechnologyensureshighspecificity
Components
  • FastÄQTMOne-StepReverseTranscriptase-Optimized50XformulationofrecombinantMMLVreversetranscriptaseforone-stepRT-PCR.
  • One-StepSYBRGreenMasterMix,ROX(2X)-2XreactionbuffercontainingdNTPs,magnesiumchloride,FastÄQTaqDNApolymerase,stABIlizers,ROXreferencedyeandSYBRGreenIdye
  • Nuclease-freewater
StorageKitcomponentsarestableforoneyearwhenstoredinaconstanttemperaturefreezerat-20°C,protectedfromlight.Forconvenience,theOne-StepSYBRGreenMasterMix,ROXmaybestoredunfrozenat+2to+8°Cforupto4months.
Shipping-20°C
NoteForresearchuseonly
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