Description
CatalogueNumber	FCCS025132
TradeName	FlowCellect
Description	FlowCellect™p38StressPathwayActivationDetectionKit
Overview	Millipore’sFlowCellect™p38StressPathwayActivationDetectionKitisdesignedtoenablearesearcheraquickandeasywaytodetectthephosphorylationstateofP38αandATF2byflowcytometry.Millipore’sFlowCellect™p38StressPathwayActivationKitwasdevelopedandtestedusingtheactivatingreagent,anisomycin,inJurkatcellsbutthekitcanbeusedwithotherhumancelllinestodeterminetheeffectofstressstimuliandchemicalreagentsthatcaninduceP38pathwaysignaling. Millipore’sFlowCellect™p38StressPathwayActivationDetectionKitcontainssufficientreagentsfor252-colortests.Thekitincludestwooptimizedfluorescentlabeledantibodiesandbuffersnecessaryforcellpreparationandanalysis.Detailedassayinstructionsareincludedtoassistinyouranalysisandtoensurethatthecorrectcellconcentrationisobtainedduringacquisitionofsampledata.
AlternateNames	FlowCellect
BackgroundInformation	p38isamemberofthemitogenactivatedkinasefamilythatrespondstostressstimuli,suchasinflammatorycytokines,ultravioletirrADIation,heatshock,andosmoticshock.p38isinvolvedincelldifferentiationandapoptosis.Morerecently,P38hasbeenshowntohavearoleincellcyclecheckpointsandDNAdamagepathways.Theupstreamkinases,MKK3,MKK6andSEKactivatep38αbyphosphorylationatThr180andTyr182. Onceactivated,p38αMAPkinasehasbeenshowntophosphorylateandactivateanumberofproteinsincludingMAPKAPkinase2andthetranscriptionfactorsATF-2,MEF2andMSK1.

ProductInformation
Components	1.20XAnti-pP38(T180/Y182)AlexaFluor®488Antibody:(PartNo.CS205783)1vialcontainingµL. 2.20XAnti-pATF2(T69/71)AlexaFluor®647Antibody:(PartNo.CS205784)1vialcontaining150µL. 3.FixationBuffer:(PartNo.CS202122)Onebottlecontaining13mL. 4.10XWashBuffer:(PartNo.CS202123)Onebottlecontaining13mL. 5.5XAssayBuffer:(PartNo.CS202124)One55mLbottle 6.1XPermeABIlizationBuffer:(PartNo.CS203284)Onebottlecontaining13mL.
Detectionmethod	Fluorescent

StorageandShippingInformation
StorageConditions	4-8°Cforantibodiesandbuffers

Applications
Application	ThisFlowCellectp38StressPathwayActivationDetectionKitforFlowCytometryisdesignedtoenablearesearcheraquick&easywaytodetectthephosphorylationstateofP38α&ATF2byflowcytometry.
KeyApplications	FlowCytometry

BIOLOGicalInformation
Host	Mouse
SpeciesReactivity	Human
AntibodyType	MonoclonalAntibody

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore Immobilon -P转印膜   PVDF膜 ·适用于免疫印迹、斑点印迹和其他蛋白质印迹的最佳PVDF膜；·Western Blotting、吸附分析、氨基酸分析、N端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析；·与他相容的染液/染色剂：考马斯亮蓝染色剂、酰胺黑、印度墨水、丽春红、胶体金、CPTS、甲苯胺蓝、透射显影和Sypro Ruby染料. 产品名称Immobilon-P说    明应用于>20KD蛋白质的Western, dot/slot blotting和其他印迹孔    径0.45um尺寸cm×cm26.5×375数    量1卷目录编号IPVH00010 ――――――――――――――――――――――――――――――――――――――― Immobilon - NC膜，混合纤维素酯（Millipore） Immobilon-NC HATF由混合纤维素酯构成，不含细胞生长期间影响细胞完整的表面活性剂。使用这些膜可从事菌落转移实验。Immobilon-NC HAHY由混合纤维素酯构成，但含有转印过程期间能改善可湿性便于操作的表面活性剂。膜的孔径是0.45μm。用于Southern blotting，Northern Blotting，Western blotting，Colony/plaque Lifts，Dot/slot blots，吸附分析，细胞培养.。 产品名称Immobilon - NC说    明应用于Southern杂交， Northern杂交，免疫印迹，结合实验孔    径0.45um尺寸cm×cm33×300数    量1卷目录编号HATF00010