Description
CatalogueNumber	07-862
BrandFamily	Upstate
TradeName	Upstate
Description	Anti-phospho-PDGFRα(Tyr754)Antibody
AlternateNames	CD140A MGC74795 PDGFR2 platelet-derivedgrowthfactorreceptor,alpha

ProductInformation
Format	AffinityPurified
Presentation	100μLofaffinitypurifiedrabbitpolyclonalIgGinbuffer(PBS(withoutMg2+andCa2+),pH7.4containing1.0mg/mLBSA(IgG,proteasefree)and0.05%sodiumazide).

StorageandShippingInformation
StorageConditions	2yearsat-80°C. Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-80°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.

Applications
Application	ThisAnti-phospho-PDGFRα(Tyr754)AntibodyisvalidatedforuseinWBforthedetectionofphospho-PDGFRα(Tyr754).
KeyApplications	WesternBlotting

BIOLOGicalInformation
Immunogen	Peptidecorrespondingtoaminoacidregionencompassingthehumanphospho-PDGFRα(Tyr754).
Host	Rabbit
Specificity	HumanandMousePDGFRαthatisphosphorylatedonTyrosine754and762,respectively.Ratispredictedtoreactbasedonsequencehomology.
Isotype	IgG
SpeciesReactivity	Human Mouse
SpeciesReactivityNote	Predictedtocross-reactwithratbasedon100%sequencesimilarity,buthasnotbeentested.
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NM_006206.3
GeneSymbol	PDGFRA Rhe-PDGFRA PDGFR2 PDGF-R-alpha CD140A CD140a MGC74795
Modifications	Phosphorylation
PurificationMethod	AffinityPurfied
UniProtNumber	Q9UD28
MolecularWeight	170-185kDA

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore Immobilon -P转印膜   PVDF膜 ·适用于免疫印迹、斑点印迹和其他蛋白质印迹的最佳PVDF膜；·Western Blotting、吸附分析、氨基酸分析、N端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析；·与他相容的染液/染色剂：考马斯亮蓝染色剂、酰胺黑、印度墨水、丽春红、胶体金、CPTS、甲苯胺蓝、透射显影和Sypro Ruby染料. 产品名称Immobilon-P说    明应用于>20KD蛋白质的Western, dot/slot blotting和其他印迹孔    径0.45um尺寸cm×cm26.5×375数    量1卷目录编号IPVH00010 ――――――――――――――――――――――――――――――――――――――― Immobilon - NC膜，混合纤维素酯（Millipore） Immobilon-NC HATF由混合纤维素酯构成，不含细胞生长期间影响细胞完整的表面活性剂。使用这些膜可从事菌落转移实验。Immobilon-NC HAHY由混合纤维素酯构成，但含有转印过程期间能改善可湿性便于操作的表面活性剂。膜的孔径是0.45μm。用于Southern blotting，Northern Blotting，Western blotting，Colony/plaque Lifts，Dot/slot blots，吸附分析，细胞培养.。 产品名称Immobilon - NC说    明应用于Southern杂交， Northern杂交，免疫印迹，结合实验孔    径0.45um尺寸cm×cm33×300数    量1卷目录编号HATF00010