Description
CatalogueNumber	AB1620
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-VimentinAntibody

ProductInformation
Format	Serum
Control	Breastcarcinomatissue,Colon,Prostate,Tonsil,Tonsilarlymphoma,Uterus,Ureter
Presentation	NeatgoatSerum.Liquidcontaining0.1%sodiumazide.

StorageandShippingInformation
StorageConditions	Maintainfor1yearat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.

Applications
Application	Anti-VimentinAntibodydetectslevelofVimentin&hasbeenpublished&validatedforuseinIC,IH,IH(P)&WB.
KeyApplications	Immunocytochemistry Immunohistochemistry Immunohistochemistry(Paraffin) WesternBlotting
ApplicationNotes	Immunohistochemistry:1:20-1:40.Showsreactivityonfrozentissuesectionsaswellasformalin-fixedparaffinembeddedtissuebyimmunoperoxidaseandimmunofluorescentassays. Immunocytochemistry Westernblot Optimalworkingdilutionsmustbedeterminedbyenduser.

BIOLOGicalInformation
Immunogen	Vimentinpurifiedfromculturedhumanforeskinfibroblasts.
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Goat
Specificity	Recognizesvimentininculturedmammaliancellsandtissuesections.AB1620specificallystainsthevimentinband(58kD)usingtotalcellortissueextractsinimmunoblotting.Noreactivitytodesmin,keratin,neurofilamentsorglialfilaments.Showswidecross-reactivityamongmammalianspeciesusingvariousimmunolabelingtechniques.
SpeciesReactivity	Human Mammals
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NM_003380.2
EntrezGeneSummary	AlongwiththeMICROFILaments(actins)andmicrotubules(tubulins),theintermediatefilamentsrepresentathirdclassofwell-characterizedcytoskeletalelements.Thesubunitsdisplayatissue-specificpatternofexpression.Desmin(MIM125660)isthesubunitspecificformuscleandvimentinthesubunitspecificformesenchymaltissue.[suppliedbyOMIM]
GeneSymbol	VIM FLJ36605 Vimentin. vimentin
PurificationMethod	Unpurified
UniProtNumber	P08670
UniProtSummary	FUNCTION:SwissProt:P08670#Vimentinsareclass-IIIintermediatefilamentsfoundinvariousnon-epithelialcells,especiallymesenchymalcells. SIZE:466aminoacids;53652Da SUBUNIT:Homopolymer.ComponentofacomplexcomposedatleastofACTB,AP2M1,AP2A1,AP2A2,MEGF10andVIM.InteractswithHCVcoreprotein. TISSUESPECIFICITY:Highlyexpressedinfibroblasts,someexpressioninT-andB-lymphocytes,andlittleornoexpressioninBurkitt"slymphomacelllines.Expressedinmanyhormone-independentmammarycarcinomacelllines. PTM:Oneofthemostprominentphosphoproteinsinvariouscellsofmesenchymalorigin.Phosphorylationisenhancedduringcelldivision,atwhichtimevimentinfilamentsaresignificantlyreorganized. SIMILARITY:SwissProt:P08670##Belongstotheintermediatefilamentfamily.
MolecularWeight	58kDa

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore Immobilon -P转印膜   PVDF膜 ·适用于免疫印迹、斑点印迹和其他蛋白质印迹的最佳PVDF膜；·Western Blotting、吸附分析、氨基酸分析、N端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析；·与他相容的染液/染色剂：考马斯亮蓝染色剂、酰胺黑、印度墨水、丽春红、胶体金、CPTS、甲苯胺蓝、透射显影和Sypro Ruby染料. 产品名称Immobilon-P说    明应用于>20KD蛋白质的Western, dot/slot blotting和其他印迹孔    径0.45um尺寸cm×cm26.5×375数    量1卷目录编号IPVH00010 ――――――――――――――――――――――――――――――――――――――― Immobilon - NC膜，混合纤维素酯（Millipore） Immobilon-NC HATF由混合纤维素酯构成，不含细胞生长期间影响细胞完整的表面活性剂。使用这些膜可从事菌落转移实验。Immobilon-NC HAHY由混合纤维素酯构成，但含有转印过程期间能改善可湿性便于操作的表面活性剂。膜的孔径是0.45μm。用于Southern blotting，Northern Blotting，Western blotting，Colony/plaque Lifts，Dot/slot blots，吸附分析，细胞培养.。 产品名称Immobilon - NC说    明应用于Southern杂交， Northern杂交，免疫印迹，结合实验孔    径0.45um尺寸cm×cm33×300数    量1卷目录编号HATF00010