Description
CatalogueNumber	MAB2130
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-ICAM-1Antibody,cloneW-CAM-1
AlternateNames	CD54

ProductInformation
Format	Ascites
Control	POSITIVECONTROL:MOLT-4,Raji,andJurkatcells.
Presentation	Liquidcontainingsodiumazide.

StorageandShippingInformation
StorageConditions	Maintainat-20°Cinundilutedaliquotsforuptooneyear.Avoidrepeatedfreeze/thawcycles.

Applications
Application	DetectICAM-1usingthisAnti-ICAM-1Antibody,cloneW-CAM-1validatedforuseinFC,IH(P).
KeyApplications	FlowCytometry Immunohistochemistry(Paraffin)
ApplicationNotes	Immunohistochemistry(formalinfixed,paraffinembeddedtissues):1:20 Flowcytometryonactivatedlymphocytes. Functionalassaysinadhesionresearch. Optimalworkingdilutionsmustbedeterminedbytheenduser.

BIOLOGicalInformation
Immunogen	RAJIcells
Clone	W-CAM-1
Host	Mouse
Specificity	ICAM-1belongstotheimmunoglobulinsuperfamily,C2subset.Itisatransmembranemoleculeof90kDwith7potentialN-glycosylationsites.Itisexpressedonrestingmonocytesandendothelialcellsandcanbeupregulatedonmanyothercells,e.g.withlymphokines,onBandTlymphocytes,thymocytes,dendriticcells,keratinocytes,chondrocytes,andepithelialcells.CD54mediatescelladhesionbindingtointegrinsCD11a/CD18(LFA-1)andtoCD11b/CD18(Mac-1).TheinteractionofCD54withLFA-1enhancesantigen-specificTcellactivation.CD54alsobindstoCD43andtoPlasmodiumfalciparum-infectederythrocytes.
Isotype	IgG
SpeciesReactivity	Human Rat
SpeciesReactivityNote	Demonstratedtoreactwithratmesenchymalstemcells.
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NM_000201.1
EntrezGeneSummary	ICAM1(CD54)istypicallyexpressedonendothelialcellsandcellsoftheimmunesystem.ICAM1bindstointegrinsoftypeCD11a/CD18,orCD11b/CD18.ICAM1isalsoexploitedbyRhinovirusasareceptor.
GeneSymbol	ICAM1 P3.58 BB2 CD54 ICAM-1
UniProtNumber	P05362
UniProtSummary	FUNCTION:SwissProt:P05362#ICAMproteinsareligandsfortheleukocyteadhesionproteinLFA-1(integrinalpha-L/beta-2).Incaseofrhinovirusinfectionactsasacellularreceptorforthevirus.Alsoactsasreceptorforsomecoxsackieviruses. SIZE:532aminoacids;57825Da SUBUNIT:Interactswithhumanherpesvirus8MIR2protein(Probable). SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein. PTM:Monoubiquitinated,whichispromotedbyMARCH9andleadstoendocytosis. SIMILARITY:SwissProt:P05362##Belongstotheimmunoglobulinsuperfamily.ICAMfamily.&Contains5Ig-likeC2-type(immunoglobulin-like)domains.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore Immobilon -P转印膜   PVDF膜 ·适用于免疫印迹、斑点印迹和其他蛋白质印迹的最佳PVDF膜；·Western Blotting、吸附分析、氨基酸分析、N端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析；·与他相容的染液/染色剂：考马斯亮蓝染色剂、酰胺黑、印度墨水、丽春红、胶体金、CPTS、甲苯胺蓝、透射显影和Sypro Ruby染料. 产品名称Immobilon-P说    明应用于>20KD蛋白质的Western, dot/slot blotting和其他印迹孔    径0.45um尺寸cm×cm26.5×375数    量1卷目录编号IPVH00010 ――――――――――――――――――――――――――――――――――――――― Immobilon - NC膜，混合纤维素酯（Millipore） Immobilon-NC HATF由混合纤维素酯构成，不含细胞生长期间影响细胞完整的表面活性剂。使用这些膜可从事菌落转移实验。Immobilon-NC HAHY由混合纤维素酯构成，但含有转印过程期间能改善可湿性便于操作的表面活性剂。膜的孔径是0.45μm。用于Southern blotting，Northern Blotting，Western blotting，Colony/plaque Lifts，Dot/slot blots，吸附分析，细胞培养.。 产品名称Immobilon - NC说    明应用于Southern杂交， Northern杂交，免疫印迹，结合实验孔    径0.45um尺寸cm×cm33×300数    量1卷目录编号HATF00010