Description
CatalogueNumber	AB19021
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-α-CateninAntibody
BackgroundInformation	Thecateninsarepolypeptidesthatbindtotheconservedcytoplasmictailofcadherinsandarerequiredforcadherinfunction(Gumbineretal.1993).a-cateninisa102kDaproteinthatisassociatedwiththecadherinindirectlythroughitsinteractionwithbeta-cateninandgamma-catenin(Fouquetetal.1992).beta-Cateninanditshomologplakoglobinassociatedirectlywiththecadherin(Butzetal.1994).Thus,thecell-celladherensjunctionisastructurecomposedofthetransmembranecadherin,whichisassociateddirectlywitheitherbeta-cateninorplakoglobin,whichinturnassoctiatesdirectlywithalpha-catenin.alpha-cateninthenmediatestheinteractionbetweenthecadherin-catenincomplexandtheactinCytoskeleton(Nagafuchietal.1994;Shahiduletal.1996).alpha-cateninhasbeencharacterizedasatumorsuppressor,suggestingthatitmayhavearegulatoryfunctioninregulationtoitsstructuralrole(Rudiger1998).

ProductInformation
Format	Serum
Presentation	Serum.Liquidcontainingnopreservatives.

StorageandShippingInformation
StorageConditions	Maintainfrozenat-20°Cinundilutedaliquotsforupto12months.

Applications
Application	Detectα-CateninusingthisAnti-α-CateninAntibodyvalidatedforuseinELISA,IP,WB,IC,IH(P).
KeyApplications	ELISA Immunoprecipitation WesternBlotting Immunocytochemistry Immunohistochemistry(Paraffin)
ApplicationNotes	Westernblot Immunocytochemistry Immunohistochemistryonformalin-fixedparaffin-embeddedtissues*:1:250-1:500*requirespressurecookerantigenretrievalmethod(Nagafuchietal.1994). Immunoprecipitation ELISA Optimalworkingdilutionsmustbedeterminedbyenduser.

BIOLOGicalInformation
Immunogen	RecombinantGST-fusionprotein(aminoterminal;576aminoacids)
Host	Rabbit
SpeciesReactivity	Canine Human Mouse
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NM_001903.2
GeneSymbol	CTNNA1 FLJ36832 alpha-E-catenin CAP102 alpha-catenin alphaE-catenin
UniProtNumber	P35221
UniProtSummary	FUNCTION:SwissProt:P35221#Associateswiththecytoplasmicdomainofavarietyofcadherins.Theassociationofcateninstocadherinsproducesacomplexwhichislinkedtotheactinfilamentnetwork,andwhichseemstobeofprimaryimportanceforcadherinscell-adhesionproperties.Mayplayacrucialroleincelldifferentiation. SIZE:906aminoacids;100071Da SUBUNIT:BindsMLLT4andF-actin(Bysimilarity).InteractsdirectlywithPSEN1andCTNNB1toformpartofthePSEN1/cadherin/cateninadhesioncomplex.InteractswithARHGAP21. SUBCELLULARLOCATION:Cytoplasm,cytoskeleton.Celljunction,adherensjunction;Cytoplasmicside.Celljunction;Cytoplasmicside.Note=Foundatcell-cellboundariesandprobablyatcell-matrixboundaries. TISSUESPECIFICITY:Expressedubiquitouslyinnormaltissues. PTM:Sumoylated. DISEASE:SwissProt:P35221#Abnormalitiesofalpha-cateninareinvolvedintheprocessofcancerinvasionandmetastasis. SIMILARITY:SwissProt:P35221##Belongstothevinculin/alpha-cateninfamily.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore Immobilon -P转印膜   PVDF膜 ·适用于免疫印迹、斑点印迹和其他蛋白质印迹的最佳PVDF膜；·Western Blotting、吸附分析、氨基酸分析、N端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析；·与他相容的染液/染色剂：考马斯亮蓝染色剂、酰胺黑、印度墨水、丽春红、胶体金、CPTS、甲苯胺蓝、透射显影和Sypro Ruby染料. 产品名称Immobilon-P说    明应用于>20KD蛋白质的Western, dot/slot blotting和其他印迹孔    径0.45um尺寸cm×cm26.5×375数    量1卷目录编号IPVH00010 ――――――――――――――――――――――――――――――――――――――― Immobilon - NC膜，混合纤维素酯（Millipore） Immobilon-NC HATF由混合纤维素酯构成，不含细胞生长期间影响细胞完整的表面活性剂。使用这些膜可从事菌落转移实验。Immobilon-NC HAHY由混合纤维素酯构成，但含有转印过程期间能改善可湿性便于操作的表面活性剂。膜的孔径是0.45μm。用于Southern blotting，Northern Blotting，Western blotting，Colony/plaque Lifts，Dot/slot blots，吸附分析，细胞培养.。 产品名称Immobilon - NC说    明应用于Southern杂交， Northern杂交，免疫印迹，结合实验孔    径0.45um尺寸cm×cm33×300数    量1卷目录编号HATF00010