Description
CatalogueNumber	MABN681
Description	Anti-TrkAAntibody,clone6B2
AlternateNames	Highaffinitynervegrowthfactorreceptor Neurotrophictyrosinekinasereceptortype1 TRK1-transformingtyrosinekinaseprotein Tropomyosin-relatedkinaseA Tyrosinekinasereceptor TyrosinekinasereceptorA Trk-A gp140trk p140-TrkA
BackgroundInformation	TrkA,apparentmolecularweight140kDa,isahighaffinitynervegrowthfactor(NGF).TheTrkproto-oncogenefamilycontainsfourmembers,TrkA,TrkB,TrkC,andTrkE,whicharevariablyexpressedthroughoutthecentralandperipheralnervoussystems.TrkAbindstonervegrowthfactor(NGF)andautophosphorylatesontyrosineresidues(Tyr490,Tyr674,Tyr675,Tyr751andTyr785)toactivatemultipledownstreameffectorproteins.PhosphorylationatTyr490isrequiredforShcassociationandsubsequentactivationoftheRas-MAPkinase-signalingcascade,whichleadstoactivationofElk-1-dependentgenetranscriptionandneuritegrowth.PhosphorylationsatTyr674andTyr675liewithinthecatalyticdomainofTrkAtyrosinekinaseandreflectTrkkinaseactivity.Additionally,phosphorylationatTyr751isrequiredforPI3-kinaseassociationandactivationoftheAktsignalingcascade.

ProductInformation
Format	Ascites
Control	Humanbraintissuelysate
Presentation	MousemonoclonalIgG2basciticfluidcontainingupto0.1%sodiumazide.

StorageandShippingInformation
StorageConditions	Stablefor1yearat-20°Cfromdateofreceipt. HandlingRecommendations:Uponreceiptandpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.

Applications
Application	Anti-TrkAAntibody,clone6B2isahighlyspecificmousemonoclonalantibody,thattargetsTrkA&hasbeentestedinwesternblotting,IHC&ICC.
KeyApplications	WesternBlotting Immunohistochemistry Immunocytochemistry
ApplicationNotes	ImmunohistochemistryAnalysis:A1:100dilutionfromarepresentativelotdetectedTrkAinhumanbrainandratcerebellumtissues. ImmunocytochemistryAnalysis:A1:200-1,000dilutionfromarepresentativelotdetectedTrkAinPC-12cells. Optimalworkingdilutionsmustbedeterminedbyenduser.

BIOLOGicalInformation
Immunogen	PurifiedrecombinantextracellularfragmentofTrkAexpressedinHEK293cells.
Clone	6B2
Host	Mouse
Isotype	IgG1
SpeciesReactivity	Human Rat
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NP_002520
GeneSymbol	NTRK1 MTC TRK TRKA
PurificationMethod	Unpurified
UniProtNumber	P04629
MolecularWeight	~87kDaobserved.Uncharacterizedbandsmayappearinsomelysate(s).Uniprotdescribes4isoformsrangingbetween~77kDaand~87kDa.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore Immobilon -P转印膜   PVDF膜 ·适用于免疫印迹、斑点印迹和其他蛋白质印迹的最佳PVDF膜；·Western Blotting、吸附分析、氨基酸分析、N端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析；·与他相容的染液/染色剂：考马斯亮蓝染色剂、酰胺黑、印度墨水、丽春红、胶体金、CPTS、甲苯胺蓝、透射显影和Sypro Ruby染料. 产品名称Immobilon-P说    明应用于>20KD蛋白质的Western, dot/slot blotting和其他印迹孔    径0.45um尺寸cm×cm26.5×375数    量1卷目录编号IPVH00010 ――――――――――――――――――――――――――――――――――――――― Immobilon - NC膜，混合纤维素酯（Millipore） Immobilon-NC HATF由混合纤维素酯构成，不含细胞生长期间影响细胞完整的表面活性剂。使用这些膜可从事菌落转移实验。Immobilon-NC HAHY由混合纤维素酯构成，但含有转印过程期间能改善可湿性便于操作的表面活性剂。膜的孔径是0.45μm。用于Southern blotting，Northern Blotting，Western blotting，Colony/plaque Lifts，Dot/slot blots，吸附分析，细胞培养.。 产品名称Immobilon - NC说    明应用于Southern杂交， Northern杂交，免疫印迹，结合实验孔    径0.45um尺寸cm×cm33×300数    量1卷目录编号HATF00010