Description
CatalogueNumber	MABD99
Description	Anti-Nkx2.2Antibody,clone1C11
AlternateNames	HomeoboxproteinNkx-2.2 HomeoboxproteinNK-2homologB
BackgroundInformation	HomeoboxproteinNkx2.2isaNK2familytranscriptionfactorthatcontainsa60AA(128to187)DNAbindinghomeobox.Nkx2.2ispartofahomeodomainproteincombinatorialcodethatspecifiesProgenitorcellidentityandneuronalfateintheventralneuraltube.Nkx2-2isrequiredfortheestablishmentofthedopaminergicprogenitordomaininmammalianventralmidbrainandSEROtonergic(5-HT)neuronsinthebrainstemandventralspinalcord.Nkx2.2isselectivelyexpressedinoligodendrocyteprogenitors(OPC)thatarisefromglialprecursorslocatedintheventralneuraltube.StudieshaveshownNkx2.2isakeyregulatorofoligodendrocytedifferentiationbyregulatingdifferentiationand/ormaturation,butnottheinitialspecificationofoligodendrocyteprogenitors.

ProductInformation
Format	Purified
Control	MouseE13-14braintissuelysate
Presentation	PurifiedmousemonoclonalIgG2aκinbuffercontainingPBSwith0.05%sodiumazide.

StorageandShippingInformation
StorageConditions	Stablefor1yearat2-8°Cfromdateofreceipt.

Applications
Application	Anti-Nkx2.2Antibody,clone1C11isahighlyspecificmousemonoclonalantibody&thattargetsNKX2.2&hasbeentestedinwesternblotting.
KeyApplications	WesternBlotting

BIOLOGicalInformation
Immunogen	RecombinantproteincorrespondingtohumanNkx2.2.
Clone	1C11
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Mouse
Isotype	IgG2aκ
SpeciesReactivity	Mouse
SpeciesReactivityNote	DemonstratedtoreactwithMouse. PredictedtoreactwithHumanbasedonimmunogendesign.
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NP_002500
EntrezGeneSummary	Theproteinencodedbythisgenecontainsahomeoboxdomainandmaybeinvolvedinthemorphogenesisofthecentralnervoussystem.Thisgeneisfoundonchromosome20nearNKX2-4,andthesetwogenesappeartobeduplicatedonchromosome14intheformofTITF1andNKX2-8.Theencodedproteinislikelytobeanucleartranscriptionfactor.[providedbyRefSeq,Jul2008].
GeneSymbol	NKX2-2 NKX2.2 NKX2B
PurificationMethod	ProteinGPurified
UniProtNumber	O95096
UniProtSummary	FUNCTION:Actsasatranscriptionalactivator.RequiredforthemaintenanceofNEUROD1expressioninthehoromone-producingendocrinecellsofthepancreas.Maybeinvolvedinspecifyingdiencephalicneuromericboundaries,andincontrollingtheexpressionofgenesthatplayaroleinaxonalguidance.AssociateswithchromatinattheNEUROD1promoterregion.BindstoasubsetofconsensuselementswithintheNEUROD1promoter(Bysimilarity). SUBUNITSTRUCTURE:InteractswithOLIG2(Bysimilarity). SUBCELLULARLOCATION:Nucleus(Bysimilarity). DOMAIN:ThehomeodomainisessentialforinteractionwithOLIG2(Bysimilarity). SEQUENCESIMILARITIES:BelongstotheNK-2homeoboxfamily. Contains1homeoboxDNA-bindingdomain.
MolecularWeight	~37kDaobserved. Uncharacterizedbandsmayappearat~25kDaand~300kDainsomelysates.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore Immobilon -P转印膜   PVDF膜 ·适用于免疫印迹、斑点印迹和其他蛋白质印迹的最佳PVDF膜；·Western Blotting、吸附分析、氨基酸分析、N端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析；·与他相容的染液/染色剂：考马斯亮蓝染色剂、酰胺黑、印度墨水、丽春红、胶体金、CPTS、甲苯胺蓝、透射显影和Sypro Ruby染料. 产品名称Immobilon-P说    明应用于>20KD蛋白质的Western, dot/slot blotting和其他印迹孔    径0.45um尺寸cm×cm26.5×375数    量1卷目录编号IPVH00010 ――――――――――――――――――――――――――――――――――――――― Immobilon - NC膜，混合纤维素酯（Millipore） Immobilon-NC HATF由混合纤维素酯构成，不含细胞生长期间影响细胞完整的表面活性剂。使用这些膜可从事菌落转移实验。Immobilon-NC HAHY由混合纤维素酯构成，但含有转印过程期间能改善可湿性便于操作的表面活性剂。膜的孔径是0.45μm。用于Southern blotting，Northern Blotting，Western blotting，Colony/plaque Lifts，Dot/slot blots，吸附分析，细胞培养.。 产品名称Immobilon - NC说    明应用于Southern杂交， Northern杂交，免疫印迹，结合实验孔    径0.45um尺寸cm×cm33×300数    量1卷目录编号HATF00010