Description
CatalogueNumber	04-218
BrandFamily	Upstate
TradeName	Upstate
Description	Anti-CREBAntibody,cloneE306,rabbitmonoclonal
AlternateNames	cAMPresponsiveelementbindingprotein activetranscriptionfactorCREB transactivatorprotein
BackgroundInformation	CREB,cyclicAMP(cAMP)responseelement-bindingprotein,isaubiquitouslyexpressedbasicleucinezippertranscriptionfactorthatfunctionsinglucosehomeostasisandstimulatestheexpressionofnumerousgenesinresponsetogrowthfactors,hormones,neurotransmitters,ionfluxes,stresssignals,andhasbeenimplicatedinmemorybecauseifitsapparentroleinsynapticplasticity.CREBfunctionseitherbyitselforwithrelatedfamilymembers,whichincludeCREB,CREM,andATF-1,byforminghomo-andheterodimersthroughtheirbasicleucinezipper(bZIP)domains.TheCREBsignalingpathwayisactivatedbyextracellularligandsthatbindtocellsurfacereceptorsandrelaytheirmessagethroughvariousintracellularsecondmessengersresultingultimatelyintheactivationofnuclearCREBthroughphosphorylationofthetransactivationdomainatserine1332,4.TheclassicalmodelofCREBactivationisthroughPKA(cAMP-dependentproteinkinase).Inthenon-activestate,PKAresidesinthecytoplasmasaninactiveheterotetramerofpairedregulatoryandcatalyticsubunits.StimulationofcAMPcausesthereleasesofthecatalyticsubunitsofPKAthatpassivelydiffuseintothenucleusandinitiatesgeneexpressionbyphosphorylatingCREBatitstransactivationdomainonserine1331,2,3,4.CREBmediatestheactivationofcAMP-responsivegenesbybindingasadimertoaconservedcAMP-responsiveelement(CRE),5’-TGACGTCA-3’,locatedwithinthepromoterregionoftargetgenes.Targetgeneactivationisterminatedbytheserine/threoninephosphatasesPP-1-mediateddephospho-rylationofCREB.

ProductInformation
Format	CultureSupernatant
Control	A431wholecelllysate.
Presentation	100μLofrabbitmonoclonalIgGin60%storagebuffer(50mMTris-Glycine(pH7.4),0.15MNaCl,0.01%sodiumazideand0.05%BSA)and40%glycerol.

StorageandShippingInformation
StorageConditions	Stablefor1yearat-20ºCfromdateofreceipt. HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.

Applications
Application	PleasenotethatthisproductwillnotbeavailableforsaleafterMarch15,2015.Pleaseselectoneoftheotherantibodiesagainstthistarget.Anti-CREBAntibody,cloneE306,rabbitdetectslevelofCREB&hasbeenpublished&validatedforuseinFC,IC,IF,IH(P),IP&WB.
KeyApplications	FlowCytometry Immunocytochemistry Immunofluorescence Immunohistochemistry(Paraffin) Immunoprecipitation WesternBlotting
ApplicationNotes	FlowCytometry:1:40dilutionfromapreviouslotwasusedonflowcytometry. Immunohistochemistry:1:250-500dilutionfromapreviouslotwasusedonimmunohistochemistry. Immunoprecipitation:1:80dilutionfromapreviouslotwasusedonimmunoprecipitation. Immunocytochemistry:1:250-500dilutionfromapreviouslotwasusedonimmunocytochemistry.

BIOLOGicalInformation
Immunogen	KLHconjugatedtoCREBlinearpeptide.
Epitope	Internal
Clone	E306
Host	Rabbit
Specificity	ThisantibodyrecognizesCREB.
Isotype	IgG
SpeciesReactivity	Human Mouse Rat
SpeciesReactivityNote	Proventoreactwithhuman,ratandmouse.
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NM_004379
GeneSymbol	MGC9284 CREB
UniProtNumber	P16220
UniProtSummary	Function:ThisproteinbindsthecAMPresponseelement(CRE),asequencepresentinmanyviralandcellularpromoters.CREBstimulatestranscriptiononbindingtotheCRE.TransciptionactivationisenhancedbytheTORCcoactivatorswhichactindependentlyofSer-133phosphorylation.ImplicatedinsynchronizationofcircADIanrhythmicity. Subunitstructure:InteractswithPPRC1.BindsDNAasadimer.Thisdimerisstabilizedbymagnesiumions.Interacts,throughthebZIPdomain,withthecoactivatorsTORC1/CRTC1,TORC2/CRTC2andTORC3/CRTC3.WhenphosphorylatedonSer-133,bindsCREBBPBysimilarity. Subcellularlocation:Nucleus. Post-translationalmodification:Stimulatedbyphosphorylation.PhosphorylationofbothSer-133andSer-142intheSCNregulatestheactivityofCREBandparticipatesincircadianrhythmgeneration.PhosphorylationofSer-133allowsCREBBPbindingBysimilarity.PhosphorylateduponDNAdamage,probablybyATMorATR. Sequencesimilarities:BelongstothebZIPfamily. Contains1bZIPdomain. Contains1KID(kinase-inducIBLe)domain.
MolecularWeight	~43kDa

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore Immobilon -P转印膜   PVDF膜 ·适用于免疫印迹、斑点印迹和其他蛋白质印迹的最佳PVDF膜；·Western Blotting、吸附分析、氨基酸分析、N端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析；·与他相容的染液/染色剂：考马斯亮蓝染色剂、酰胺黑、印度墨水、丽春红、胶体金、CPTS、甲苯胺蓝、透射显影和Sypro Ruby染料. 产品名称Immobilon-P说    明应用于>20KD蛋白质的Western, dot/slot blotting和其他印迹孔    径0.45um尺寸cm×cm26.5×375数    量1卷目录编号IPVH00010 ――――――――――――――――――――――――――――――――――――――― Immobilon - NC膜，混合纤维素酯（Millipore） Immobilon-NC HATF由混合纤维素酯构成，不含细胞生长期间影响细胞完整的表面活性剂。使用这些膜可从事菌落转移实验。Immobilon-NC HAHY由混合纤维素酯构成，但含有转印过程期间能改善可湿性便于操作的表面活性剂。膜的孔径是0.45μm。用于Southern blotting，Northern Blotting，Western blotting，Colony/plaque Lifts，Dot/slot blots，吸附分析，细胞培养.。 产品名称Immobilon - NC说    明应用于Southern杂交， Northern杂交，免疫印迹，结合实验孔    径0.45um尺寸cm×cm33×300数    量1卷目录编号HATF00010