Description
CatalogueNumber	CBL468
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-PECAM-1Antibody,domains3-6ofhumanPECAM-1,cloneHC1/6
AlternateNames	CD31

ProductInformation
Format	Purified
Control	Activatedmicroglialcells
Presentation	ProteinAPurifiedmouseimmunoglobulinin20mMsodiumphosphate,250mMNaCl,pH.7.6,with0.1%sodiumazideasapreservative.

StorageandShippingInformation
StorageConditions	Forusewithin1monthofpurchasestoreat+4°C,forlongtermstoragealiquotantibodyintosmallvolumesandstoreat-20°Cforuptooneyear.

Applications
Application	Anti-PECAM-1Antibody,domains3-6ofhumanPECAM-1,cloneHC1/6isanantibodyagainstPECAM-1foruseinFC,IH,IH(P)&IP.
KeyApplications	FlowCytometry Immunohistochemistry Immunohistochemistry(Paraffin) Immunoprecipitation
ApplicationNotes	StudiesoftheroleofCD31inleucocytemigrationintoinflammatorysitesandintheprocessofangiogenesis Thisantibodywillstainfrozentissuesections Suitableforimmunoprecipitationstudies SuitableforflowcytometryeitherindirectlyordirectlywhenlabelledwithFITCorR-PE Suitableforuseonformalinfixedparaffinembeddedsectionsafterproteasepre-treatment. Optimalworkingdilutionsmustbedeterminedbytheenduser.

BIOLOGicalInformation
Immunogen	PMA-treatedU937cells
Epitope	domains3-6ofhumanPECAM-1
Clone	HC1/6
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Mouse
Specificity	Reactswitha130-140kDatransmembranousglycoproteinthatisexpressedwidelyamongstcellsofthevascularcompartment.CD31isexpressedonplatelets,culturedendothelialcells,monocytes,normaltissuemacrophagesintonsil,lungandkidneyaswellasinskinbiopsiesfromconditionssuchassarcoidosisandlepromatousleprosy. FUSIONPARTNER:P3-x63Ag8.653myelomacellline
Isotype	IgG1
SpeciesReactivity	Human
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NM_000442.3
GeneSymbol	PECAM1 EndoCAM GPIIA PECAM-1 CD31 CD31/EndoCAM
PurificationMethod	ProteinAPurfied
UniProtNumber	P16284
UniProtSummary	FUNCTION:SwissProt:P16284#Thisproteinisacelladhesionmoleculeexpressedonplateletsandatendothelialcellintercellularjunctions. SIZE:738aminoacids;82536Da SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein. TISSUESPECIFICITY:Longisoformpredominatesalltissuesexamined,isoformDelta12wasdetectedonlyintracheaandisoformDelta14-15onlyinlung,isoformDelta14wasdetectedinalltissuesexaminedwiththestrongestexpressioninheart. PTM:PhosphorylatedonSerandTyrresiduesaftercellularactivation. SIMILARITY:SwissProt:P16284##Contains6Ig-likeC2-type(immunoglobulin-like)domains.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore Immobilon -P转印膜   PVDF膜 ·适用于免疫印迹、斑点印迹和其他蛋白质印迹的最佳PVDF膜；·Western Blotting、吸附分析、氨基酸分析、N端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析；·与他相容的染液/染色剂：考马斯亮蓝染色剂、酰胺黑、印度墨水、丽春红、胶体金、CPTS、甲苯胺蓝、透射显影和Sypro Ruby染料. 产品名称Immobilon-P说    明应用于>20KD蛋白质的Western, dot/slot blotting和其他印迹孔    径0.45um尺寸cm×cm26.5×375数    量1卷目录编号IPVH00010 ――――――――――――――――――――――――――――――――――――――― Immobilon - NC膜，混合纤维素酯（Millipore） Immobilon-NC HATF由混合纤维素酯构成，不含细胞生长期间影响细胞完整的表面活性剂。使用这些膜可从事菌落转移实验。Immobilon-NC HAHY由混合纤维素酯构成，但含有转印过程期间能改善可湿性便于操作的表面活性剂。膜的孔径是0.45μm。用于Southern blotting，Northern Blotting，Western blotting，Colony/plaque Lifts，Dot/slot blots，吸附分析，细胞培养.。 产品名称Immobilon - NC说    明应用于Southern杂交， Northern杂交，免疫印迹，结合实验孔    径0.45um尺寸cm×cm33×300数    量1卷目录编号HATF00010