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- Waschk,D.E. etal. (2011) Am.J.Physiol.Cell.Physiol. 300, C843.
AlomoneLabs1-EBIOactivatesKCa3.1currentsexpressedinHEK293Tcells.A.TimecourseofKCa3.1currentamplitudeanditsactivationby50µM 1-EBIO (#E-150).Currentswereelicitedbyapplicationofvoltagerampfrom-120mVto+60mV(150msec),deliveredevery10seconds,holdingpotentialof-80mV.B.Superimposedexampletracesofcurrentresponsesbeforeandduringperfusionof50µM1-EBIOasindicated.
- 1.Wong, R. etal. (2014) J.Neurosci. 34, 13371.
- 2.Coleman,N. etal. (2014) Mol.Pharmacol. 86, 342.
- 3.Strøbæk, D. etal. (2013) Br.J.Pharmacol. 168, 432.
1-EBIOisapotentKCa2/3channelactivatorthatactivatesthechannelsbyincreasingtheCa2+sensitivitybystABIlizingtheinteractionbetweencalmodulinandthechannels1,2,thusshiftingtheactivationcurveofthechannelstotheleft.1-EBIOactivatesKCa3.1withanEC50valueofaround30μMandallthreeKCa2channelswithEC50valuearound300μM2.
TheCa2+-activatedK+channel,KCa3.1,playsamajorroleinthecontrolledexpressionofessentialgenesthatareresponsIBLeforseveralphysiologicalprocessesincluding-membranepotential,cellvolumeproliferation,migrationandproductionofinflammatorymediatorsbyparticipatingintheregulationofcalciumentry.AhighlyregulatedandsustainedincreaseincytosolicCa2+iscriticalintheseprocessesmakingKCa3.1apotentialtargetfortherapyofseveraldisease1,3.
1-EBIO(#E-150) isahighlypure,synthetic,andbiologicallyactivecompound.



