发现抗体/抗水通道蛋白2-ATTO-550抗体/50µl/AQP-002-AO-50µl

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¥10540.00
货号:AQP-002-AO-50µl
浏览量:127
品牌:Discovery
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商品描述
Cat#:AQP-003-AR
AlternativeNameAQP3,Aquaglyceroporin-3,GIL,GLIP
  • LyophilizedPowder
  • AntigenIncl.
  • Type:Polyclonal
    Host:Rabbit
    Reactivity:h,m,r
      • Peptide(C)STEAENVKLAHMKHKEQI,correspondingtoaminoacidresidues275-292ofratAQP3(Accession P47862).Intracellular,C-terminus.
        Anti-Aquaporin 3-ATTO-594 Antibody
    Accession(Uniprot)NumberP47862
    GeneID65133
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyMouse-17/18aminoacidresiduesidentical;sheep,human-15/18aminoacidresiduesidentical.
    RRIDAB_2039732.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    IsotypeRabbitIgG.
    LabelATTO-594.Maximumabsorption601nm;maximumfluorescence627nm.Thefluorescenceisexcitedmostefficientlyinthe580-615nmrange.ThislabelisrelatedtotheRhodaminedyesandcanbeusedwithfiltersusedtodetectTexasRedandAlexa-594.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution50µldoubledistilledwater(DDW).
    Antibodyconcentrationafterreconstitution1mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).
    NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).
    Negativecontrolantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1μgpeptideper1μgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AQP-003),andimmunohistochemistry(labeledantibody).
    Applications:if,ih
    Mayalsoworkin:ic*
      • Anti-Aquaporin 3-ATTO-594 Antibody
        ExpressionofAquaporin3inratcolon
        Immunohistochemicalstainingofratcolonparaffin-embeddedsections using Anti-Aquaporin3-ATTO-594Antibody(#AQP-003-AR),(1:100).Staining(red)ispresentinabsorptivecellsofthecolonicepithelium.Hoechst33342isusedascounterstain(blue).
        Anti-Aquaporin 3-ATTO-594 Antibody
        ExpressionofAquaporin3inratkidney
        Immunohistochemicalstainingofratkidneyparaffin-embeddedsectionsusing Anti-Aquaporin3-ATTO-594Antibody(#AQP-003-AR),(1:100).A.Staining(red)ispresentincollectingductsinthecorticomedullaryjunction.B.Highmagnificationconfocalimageshowingstainingindiscretecollectingductscells.Hoechst33342 isusedascounterstain(blue).
    References
    • 1.King,L.S.etal.(2004)Nat.Rev.Mol.CellBiol.5,687.
    • 2.Hara,M.etal.(2003)Proc.Natl.Acad.Sci.U.S.A.100,7360.
    • 3.Hara-Chikuma,M.andVerkman,A.S.(2008)Mol.Cell.Biol.28,326.
      • Aquaporin3(AQP-3)belongstoafamilyofmembraneproteinsthatallowpassageofwaterandcertainothersolutesthroughBIOLOGicalmembranes.Thefamilyiscomposedof13members(AQP-0toAQP-12).

        The aquaporins canbedividedintotwofunctionalgroupsbasedontheirpermABIlitycharacteristics:theaquaporinsthatareonlypermeatedbywaterandtheaquaglyceroporinsthatarepermeatedbywaterandothersmallsolutessuchasglycerol.ThislastgroupincludesAQP-3aswellas AQP-7, AQP-9 andAQP-10.1 Littleisknownaboutthefunctionofthetwonewestmembers, AQP-11 andAQP-12.  

        TheproteinspresentaconservedstructureofsixtransmembranedomainswithintracellularN-andC-termini.Thefunctionalchannelisatetramerbuteachsubunithasaseparateporeandthereforethefunctionalchannelunit,containsfourpores.1

        AQP-3iswidelyexpressedinseveralorganswithprominentexpressionfoundintheskin,colon,lungandkidney.ConsistentwithacentralfunctionofAQP-3inskin,micedeficientinAQP-3havereducedskinwatercontentandelasticitycomparedwithwild-typemice,aswellasimpairedwoundhealingandepidermalbiosynthesis.2 FurThermore,AQP-3deficientmicewerefoundtoberesistanttoskintumordevelopmentsuggestingaroleforthisaquaporinintumOrigenesis.3

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IF-Immunofluorescence,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    Lastupdate:10/11/2019

    Anti-Aquaporin3Antibody(#AQP-003)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblot,immunoprecipitation,indirectflowcytometry,andimmunohistochemistryapplications.IthasbeendesignedtorecognizetheAQP3channelfromrat,human,andmousesamples.

    Anti-Aquaporin3-ATTO-594Antibody(#AQP-003-AR)isdirectlylabeledwithanATTO-594fluorescentdye.ATTOdyesarecharacterizedbystrongabsorption(highextinctioncoefficient),highfluorescencequantumyield,andhighphoto-stability.TheATTO-594fluorescentlabelbelongstotheclassofRhodaminedyesandcanbeusedwithfluorescentequipmenttypicallyoptimizedtodetectTexasRedandAlexa-594.Anti-Aquaporin3-ATTO-594Antibody isespeciallysuitedforexperimentsrequiringsimultaneouslabelingofdifferentMarkers.

    Forresearchpurposesonly,notforhumanuse
    Discovery®抗体是Cambridge Research Biochemicals的目录抗体业务。 CRB是全球第二大最古老的肽公司,也是抗体研究市场的先驱,目前正以Discovery®Antibodies的名义建立一系列新验证的靶标抗体。 Discovery®抗体将专注于研究用抗体(早期药物“发现”),并将补充我们非常成功且长期存在的定制抗体业务(www.crbdiscovery.com)。我们已经以新颖,复杂,稀有和翻译后修饰的特异性抗体而闻名,这些受追捧的靶标将出现在我们的Discovery®Antibody目录范围内。  我们于2017年2月,在发现肽之后一年,并且在詹姆斯·库克船长(他当时最伟大的探险家)于1776年从英国出发,乘坐“ HMS发现”号开始租用新水域和“发现全球各地的新土地。因此,“发现”这个名称似乎适合东北制造商–詹姆斯·库克的故乡。 在1700年代后期对全球新发现土地的追求与医学研究的新“发现”以及2000年人类基因组图谱的发现之间,还有其他相似之处。  有关更多详细信息,请参见有关Discovery®抗体发布的新闻稿。   Cambridge Research Biochemicals  (www.crbdiscovery.com)成立于1980年,是英国领先的定制肽和抗体工具的独立生产商,为全球制药,生命科学和学术领域的研究人员提供产品。其产品的主要应用是定制的实验室规模试剂,用于早期药物发现中的主要研究证据,并支持临床研究中的生物标志物计划。该公司生产各种口味的肽,从简单到修饰,稳定和放射性标记,再到从毫克到克到最高纯度的染料标记。此外,CRB是产生高度特异性和靶向抗体(多克隆抗体和单克隆抗体)的公认专家。荧光染料标记也是重点。自1994年以来,该公司于2016年启动了其首个目录业务DISCOVERY®。
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