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LyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!
- Peptide(C)STEAENVKLAHMKHKEQI,correspondingtoaminoacidresidues275-292ofratAQP3(Accession P47862).Intracellular,C-terminus.

ExpressionofAquaporin3inratcolonImmunohistochemicalstainingofratcolonparaffin-embeddedsections using Anti-Aquaporin3-ATTO-594Antibody(#AQP-003-AR),(1:100).Staining(red)ispresentinabsorptivecellsofthecolonicepithelium.Hoechst33342isusedascounterstain(blue).
ExpressionofAquaporin3inratkidneyImmunohistochemicalstainingofratkidneyparaffin-embeddedsectionsusing Anti-Aquaporin3-ATTO-594Antibody(#AQP-003-AR),(1:100).A.Staining(red)ispresentincollectingductsinthecorticomedullaryjunction.B.Highmagnificationconfocalimageshowingstainingindiscretecollectingductscells.Hoechst33342 isusedascounterstain(blue).
- 1.King,L.S.etal.(2004)Nat.Rev.Mol.CellBiol.5,687.
- 2.Hara,M.etal.(2003)Proc.Natl.Acad.Sci.U.S.A.100,7360.
- 3.Hara-Chikuma,M.andVerkman,A.S.(2008)Mol.Cell.Biol.28,326.
Aquaporin3(AQP-3)belongstoafamilyofmembraneproteinsthatallowpassageofwaterandcertainothersolutesthroughBIOLOGicalmembranes.Thefamilyiscomposedof13members(AQP-0toAQP-12).
The aquaporins canbedividedintotwofunctionalgroupsbasedontheirpermABIlitycharacteristics:theaquaporinsthatareonlypermeatedbywaterandtheaquaglyceroporinsthatarepermeatedbywaterandothersmallsolutessuchasglycerol.ThislastgroupincludesAQP-3aswellas AQP-7, AQP-9 andAQP-10.1 Littleisknownaboutthefunctionofthetwonewestmembers, AQP-11 andAQP-12.
TheproteinspresentaconservedstructureofsixtransmembranedomainswithintracellularN-andC-termini.Thefunctionalchannelisatetramerbuteachsubunithasaseparateporeandthereforethefunctionalchannelunit,containsfourpores.1
AQP-3iswidelyexpressedinseveralorganswithprominentexpressionfoundintheskin,colon,lungandkidney.ConsistentwithacentralfunctionofAQP-3inskin,micedeficientinAQP-3havereducedskinwatercontentandelasticitycomparedwithwild-typemice,aswellasimpairedwoundhealingandepidermalbiosynthesis.2 FurThermore,AQP-3deficientmicewerefoundtoberesistanttoskintumordevelopmentsuggestingaroleforthisaquaporinintumOrigenesis.3
Anti-Aquaporin3Antibody(#AQP-003)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblot,immunoprecipitation,indirectflowcytometry,andimmunohistochemistryapplications.IthasbeendesignedtorecognizetheAQP3channelfromrat,human,andmousesamples.
Anti-Aquaporin3-ATTO-594Antibody(#AQP-003-AR)isdirectlylabeledwithanATTO-594fluorescentdye.ATTOdyesarecharacterizedbystrongabsorption(highextinctioncoefficient),highfluorescencequantumyield,andhighphoto-stability.TheATTO-594fluorescentlabelbelongstotheclassofRhodaminedyesandcanbeusedwithfluorescentequipmenttypicallyoptimizedtodetectTexasRedandAlexa-594.Anti-Aquaporin3-ATTO-594Antibody isespeciallysuitedforexperimentsrequiringsimultaneouslabelingofdifferentMarkers.


