发现抗体/抗CALHM1抗体/50µl/ACC-101-50µl

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¥7340.00
货号:ACC-101-50µl
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品牌:Discovery
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商品描述
Cat#:ACC-101
AlternativeNameCalciumhomeostasismodulator1,FAM26C
  • LyophilizedPowder
  • AntigenIncl.
  • Type:Polyclonal
    Host:Rabbit
    Reactivity:h,m,r
      • Peptide(C)EAMNHDLELGHTHG,correspondingtoaminoacidresidues252-265ofhumanCALHM1(Accession Q8IU99).Intracellular,C-terminus.
        Anti-CALHM1 Antibody
    Accession(Uniprot)NumberQ8IU99
    GeneID255022
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyRat,mouse-identical.
    RRIDAB_11125728.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    IsotypeRabbitIgG.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
    Antibodyconcentrationafterreconstitution0.8mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
    NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).
    Negativecontrolantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1µgpeptideper1µgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis.
    Applications:if,ih,wb
    Mayalsoworkin:ic*,ifc*,ip*
      • Anti-CALHM1 Antibody
        Westernblotanalysisofratbrain(lanes1and4),mousebrain(lanes2and5),U-87MG(lanes3and6)andC6(lanes4and8)lysates:
        1.Anti-CALHM1Antibody (#ACC-101),(1:200).
        2.Anti-CALHM1Antibody,preincubatedwiththenegativecontrolantigen.
      • Anti-CALHM1 Antibody
        Expressionof CALHM1inrathippocampus
        ImmunohistochemicalstainingofrathippocampususingAnti-CALHM1Antibody (#ACC-101).A.Stainingof CALHM1appearsonlyinthehippocampalCA1pyramidallayer(red).B.NuclearstainingusingDAPIasthecounterstain(blue).C.MergeofAandB.
    References
    • 1.Green,K.N.andLaFerla,F.M.(2008)Neuron59,190.
    • 2.Koppel,J.etal.(2011)Mol.Med.17,974.
    • 3.Dreses-Werringloer,U.etal.(2008)Cell133,1149.
      • AmisbalanceinCa2+ homeostasisseemstoberelatedtothedevelopmentofAlzheimer’sdisease(AD).Indeed,neuronalCa2+ balancemayaffectthelevelsofproteinsassociatedwithAD,suchasamyloid-beta(Ab)andtau1.

        Calciumhomeostasismodulator1(CALHM1)isacerebralCa2+ channelcomponentresponsIBLeforcontrollingintracellularCa2+ levelsandAbmetabolism2,3.CALHM1isathreetransmembraneglycoprotein,mostlylocalizedtotheendoplasmicreticulumalthoughitcanbedetectedattheplasmamembrane.AfunctionalchannelisformedbymultiplesubunitswhichhassomestructuralsimilaritiestotheNMDAreceptor.Itisexpressedinallbrainregions1.

        Inculturedcells,overexpressionofCALHM1increasesintracellularCa2+ levelsandreducesAbaccumulation,therebyreinforcingitsimplicationinthedevelopmentofAD.Indeed,apolymorphisminthegenewasfoundtoaffecttheonsetofAD2.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IF-Immunofluorescence,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    receivea50µlfreetrialsample!
    Lastupdate:10/11/2019

    Anti-CALHM1Antibody(#ACC-101)isahighlyspecificantibodydirectedagainstanepitopeofthehumancalciumhomeostasismodulatorprotein1.Theantibodycanbeusedinwesternblotandimmunohistochemistryapplications.IthasbeendesignedtorecognizeCALHM1fromrat,mouseandhumansamples.

    Forresearchpurposesonly,notforhumanuse
    Discovery®抗体是Cambridge Research Biochemicals的目录抗体业务。 CRB是全球第二大最古老的肽公司,也是抗体研究市场的先驱,目前正以Discovery®Antibodies的名义建立一系列新验证的靶标抗体。 Discovery®抗体将专注于研究用抗体(早期药物“发现”),并将补充我们非常成功且长期存在的定制抗体业务(www.crbdiscovery.com)。我们已经以新颖,复杂,稀有和翻译后修饰的特异性抗体而闻名,这些受追捧的靶标将出现在我们的Discovery®Antibody目录范围内。  我们于2017年2月,在发现肽之后一年,并且在詹姆斯·库克船长(他当时最伟大的探险家)于1776年从英国出发,乘坐“ HMS发现”号开始租用新水域和“发现全球各地的新土地。因此,“发现”这个名称似乎适合东北制造商–詹姆斯·库克的故乡。 在1700年代后期对全球新发现土地的追求与医学研究的新“发现”以及2000年人类基因组图谱的发现之间,还有其他相似之处。  有关更多详细信息,请参见有关Discovery®抗体发布的新闻稿。   Cambridge Research Biochemicals  (www.crbdiscovery.com)成立于1980年,是英国领先的定制肽和抗体工具的独立生产商,为全球制药,生命科学和学术领域的研究人员提供产品。其产品的主要应用是定制的实验室规模试剂,用于早期药物发现中的主要研究证据,并支持临床研究中的生物标志物计划。该公司生产各种口味的肽,从简单到修饰,稳定和放射性标记,再到从毫克到克到最高纯度的染料标记。此外,CRB是产生高度特异性和靶向抗体(多克隆抗体和单克隆抗体)的公认专家。荧光染料标记也是重点。自1994年以来,该公司于2016年启动了其首个目录业务DISCOVERY®。
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