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LyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!
- Peptide(C)EAMNHDLELGHTHG,correspondingtoaminoacidresidues252-265ofhumanCALHM1(Accession Q8IU99).Intracellular,C-terminus.

Westernblotanalysisofratbrain(lanes1and4),mousebrain(lanes2and5),U-87MG(lanes3and6)andC6(lanes4and8)lysates:1.Anti-CALHM1Antibody (#ACC-101),(1:200).
2.Anti-CALHM1Antibody,preincubatedwiththenegativecontrolantigen.
Expressionof CALHM1inrathippocampusImmunohistochemicalstainingofrathippocampususingAnti-CALHM1Antibody (#ACC-101).A.Stainingof CALHM1appearsonlyinthehippocampalCA1pyramidallayer(red).B.NuclearstainingusingDAPIasthecounterstain(blue).C.MergeofAandB.
- 1.Green,K.N.andLaFerla,F.M.(2008)Neuron59,190.
- 2.Koppel,J.etal.(2011)Mol.Med.17,974.
- 3.Dreses-Werringloer,U.etal.(2008)Cell133,1149.
AmisbalanceinCa2+ homeostasisseemstoberelatedtothedevelopmentofAlzheimer’sdisease(AD).Indeed,neuronalCa2+ balancemayaffectthelevelsofproteinsassociatedwithAD,suchasamyloid-beta(Ab)andtau1.
Calciumhomeostasismodulator1(CALHM1)isacerebralCa2+ channelcomponentresponsIBLeforcontrollingintracellularCa2+ levelsandAbmetabolism2,3.CALHM1isathreetransmembraneglycoprotein,mostlylocalizedtotheendoplasmicreticulumalthoughitcanbedetectedattheplasmamembrane.AfunctionalchannelisformedbymultiplesubunitswhichhassomestructuralsimilaritiestotheNMDAreceptor.Itisexpressedinallbrainregions1.
Inculturedcells,overexpressionofCALHM1increasesintracellularCa2+ levelsandreducesAbaccumulation,therebyreinforcingitsimplicationinthedevelopmentofAD.Indeed,apolymorphisminthegenewasfoundtoaffecttheonsetofAD2.
Anti-CALHM1Antibody(#ACC-101)isahighlyspecificantibodydirectedagainstanepitopeofthehumancalciumhomeostasismodulatorprotein1.Theantibodycanbeusedinwesternblotandimmunohistochemistryapplications.IthasbeendesignedtorecognizeCALHM1fromrat,mouseandhumansamples.


