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LyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.
- PeptideCMIEWPEHPNRTYEK,correspondingtoaminoacidresidues180-194ofratNK1 receptor(Accession P14600).2ndextracellularloop.

CellsurfacedetectionofNK1RinliveintactmouseJ774macrophagecells:___ Cells.
___ Cells+rabbitIgGisotypecontrol-FITC.
___ Cells+ Anti-Neurokinin1Receptor(NK1R) (extracellular)-FITCAntibody(#ATR-001-F),(2.5µg).
CellsurfacedetectionofNK1RinliveintacthumanTHP-1monocyticleukemiacells:___ Cells.
___ Cells+rabbitIgGisotypecontrol-FITC.
___ Cells+ Anti-Neurokinin1Receptor(NK1R)(extracellular)-FITCAntibody(#ATR-001-F),(2.5µg).
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SubstanceP(SP),NeurokininA(NKA)andNeurokininB(NKB)areallpeptidesbelongingtotheTachykininproteinfamily.Thesethreepeptideswhichdemonstrateaquiteheterogeneityintheirdistributionexerttheireffectviathreereceptors:Neurokinin1-3receptors,membersoftheG-proteincoupledreceptorsuperfamily.However,Neurokinin1Receptor(NK1)preferentiallybindsSubstanceP,Neurokinin2Receptor(NK2)toNKAandNeurokinin3Receptor(NK3)toNKB1.
Neurokininreceptorsaredistinguishedbytheirseventransmembranedomains,anextracellularN-terminusandacytosolicC-terminal.Anunusualpropertyofthesereceptorsisthepresenceofintronsaspartoftheirstructuralorganization1-3.Tachykininreceptorsundergoalternativesplicing.Forexample,NK1 isdetectedwithdifferentC-terminallengths.Thelongerreceptorisoformisfoundinthebrainwhereasthetruncatedformismostlydetectedintheperiphery1,4.
Duetothebroadexpressionprofileoftachykininpeptides,theirrespectivereceptorsarealsoexpressedinasimilarfashion.NK1 iswidelyexpressedinneuronsendothelialcells,muscleandimmunesystemcells.NK2 isbroadlyexpressedintheperipheryanditsexpressioninthebrainisquiterestricted.NK3 ontheotherhandislargelyexpressedinthecentralnervoussystemandisalsodetectedintheuterus,skeletalmuscle,lungandliver1.
Neurokininreceptorshavebeenfoundinmanypathophysiologicalindicationsandhavethereforebecometargetsforthedevelopmentofpharmacologicalcompounds.Suchindicationsincludecancer,psychologicaldisorders,migraineandvariousinflammations,justtonameafew5-8.
Anti-Neurokinin1Receptor(NK1R)(extracellular)Antibody (#ATR-001)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblot,indirectlivecellflowcytometry,andlivecellimagingapplications.IthasbeendesignedtorecognizeNK1 fromrat,mouse,andhumansamples.
Anti-Neurokinin1Receptor(NK1R)(extracellular)-FITCAntibody (#ATR-001-F)is directlyconjugatedtofluoresceinisothiocyanate(FITC). The antibodycanbeusedinimmunofluorescentapplicationssuchasdirectlivecellflowcytometry.


