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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
AlternativeNameVoltage-dependentL-typecalcium channelsubunitα1C
KOValidatedKOValidated
Thespecificityofthisantibodyhasbeenvalidatedinaknockout(KO)orknockdown(KD)system.
LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
Type:Polyclonal
Host:Rabbit
Reactivity:h,m,r
- Peptide(C)TTKINMDDLQPSENEDKS,correspondingtoaminoacid residues848-865ofratCaV1.2(Accession P22002).IntracellularloopbetweendomainsIIandIII.
Accession(Uniprot)NumberP22002
GeneID24239
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyRat,mouse -8/11 aminoacidresiduesidentical.
RRIDAB_2039771.
PurityAffinitypurifiedonimmobilizedantigen.
FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
IsotypeRabbitIgG.
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution0.8mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).
Negativecontrolantigenstorageafterreconstitution-20°C.
PreadsorptionControl1µgpeptideper1µgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis.
Applications:ic,if,ifc,ih,ip,wb
Westernblotanalysisofratbrainmembranes:1. Anti-CaV1.2(CACNA1C)Antibody(#ACC-003),(1:200).
2.Anti-CaV1.2(CACNA1C)Antibody,preincubatedwiththecontrolpeptideantigen.
WesternblotanalysisofCaV1.2-transfected Xenopus oocytes(lane1)andnon-transfectedoocyteslysates(lane2):1. Anti-CaV1.2 (CACNA1C) Antibody(#ACC-003),(1:200)in CaV1.2(CACNA1C)ChannelOverexpressedMembraneFractions(LX-104).
2.Anti-CaV1.2(CACNA1C)Antibodyinnon-transfectedoocytes.Humancardiactissue(Crossman,D.J. etal. (2011) PLoSONE 6, e17901.).
- CaV1.2transfectedHEK-293cellsandmouseheartlysate(Rougier,J.S. etal. (2011) J.Biol.Chem. 286, 8829.).
ExpressionofCaV1.2inmousecerebellumImmunohistochemicalstainingofmousecerebellumwith Anti-CaV1.2 (CACNA1C) Antibody(#ACC-003).A.CaV1.2(red)appearsinPurkinjecells(horizontalarrows)andisdistributeddiffuselyinthemolecularlayer(Mol)includinginPurkinjedendrites(verticalarrows).B.StainingofPurkinjenervecellswithmouseanti-Calbindin28K (green)demonstratesthelocationofdendritesinthemolecularlayer.C.MergedimageofpanelsAandB.Mouseatrioles(1:300)(Howitt,L. etal. (2013) J.Physiol. 591, 2157.).
- Ratinsulinomacells(RIN)(Parkash,J.(2011) LifeSci. 88, 257.).Mousechromaffincells(1:200).(Perez-Alvarez,A. etal. (2011) J.NeuRochem. 116, 105.).
- Ratmastcells(RBL-2H3cells,1μg/5x105 cells).(Yoshimaru,T. etal. (2009) Mol.Immunol. 46, 1267.).Thecontrolantigenisnotsuitableforthisapplication.
References
- 1.Catterall,W.A.etal.(2003)Pharmacol.Rev.55,579.
- 2.IUPHAR
- 3.Hu,X.Q.etal.(1998)J.Biol.Chem.273,5337.
- 4.Kreuzberg,U.etal.(2000)AmJ.Physiol.278,H723.
- 5.Allard,B.etal.(2000)J.Biol.Chem.275.25556.
AllL-typecalciumchannelsareencodedbyoneoftheCaV1channelgenes.ThesechannelsplayamajorroleasaCa2+ entrypathwayinskeletal,cardiacandsmoothmusclesaswellasinneurons,endocrinecellsandpossIBLyinnon-excitablecellssuchashematopoeticandepithelialcells.AllCaV1channelsareinfluencedbydihydropyridines(DHP)andarealsoreferredtoasDHPreceptors.
WhiletheCaV1.1andCaV1.4isoformsareexpressedinrestrictedtissues(skeletalmuscleandretina,respectively),theexpressionofCaV1.2isubiquitousand CaV1.3 channelsarefoundintheheart,brainandpancreas.SeveralpeptidyltoxinsaredescribedthatarespecificL-typechannelblockers,butsofarnoselectiveblockerforoneoftheCaV1isoformshavebeendescribed.TheseincludetheMambatoxins Calcicludine (#SPC-650), Calciseptine (#C-500)and FS-2 (#F-700).
LyophilizedPowderNegativeControlAntigenIncluded
Image&Title:
ExpressionofCaV1.2inratcardiomyocytes.ImmunocytochemicalstainingofadultratcardiomyocytesusingAnti-CaV1.2(CACNA1C) Antibody(#ACC-003).CaV1.2staining(green)isobservedinaregularlyspacedarray,aswellassurfacesarcolemmalstaining.β-arrestinstainingisinred.AdaptedfromHermosilla,T.etal.(2017)Sci.Rep.7,10131.withpermissionofSPRINGERNATURE.
ExpressionofCaV1.2inratcardiomyocytes.ImmunocytochemicalstainingofadultratcardiomyocytesusingAnti-CaV1.2(CACNA1C) Antibody(#ACC-003).CaV1.2staining(green)isobservedinaregularlyspacedarray,aswellassurfacesarcolemmalstaining.β-arrestinstainingisinred.AdaptedfromHermosilla,T.etal.(2017)Sci.Rep.7,10131.withpermissionofSPRINGERNATURE.
Lastupdate:21/11/2019
Anti-CaV1.2(CACNA1C)Antibody(#ACC-003)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblot,immunoprecipitation,immunohistochemistry,immunocytochemistry,andindirectflowcytometryapplications.IthasbeendesignedtorecognizeCaV1.2frommouse,rat,andhumansamples.
Forresearchpurposesonly,notforhumanuse
Discovery®抗体是Cambridge Research Biochemicals的目录抗体业务。 CRB是全球第二大最古老的肽公司，也是抗体研究市场的先驱，目前正以Discovery®Antibodies的名义建立一系列新验证的靶标抗体。 Discovery®抗体将专注于研究用抗体（早期药物“发现”），并将补充我们非常成功且长期存在的定制抗体业务（www.crbdiscovery.com）。我们已经以新颖，复杂，稀有和翻译后修饰的特异性抗体而闻名，这些受追捧的靶标将出现在我们的Discovery®Antibody目录范围内。 我们于2017年2月，在发现肽之后一年，并且在詹姆斯·库克船长（他当时最伟大的探险家）于1776年从英国出发，乘坐“ HMS发现”号开始租用新水域和“发现全球各地的新土地。因此，“发现”这个名称似乎适合东北制造商–詹姆斯·库克的故乡。 在1700年代后期对全球新发现土地的追求与医学研究的新“发现”以及2000年人类基因组图谱的发现之间，还有其他相似之处。 有关更多详细信息，请参见有关Discovery®抗体发布的新闻稿。 Cambridge Research Biochemicals （www.crbdiscovery.com）成立于1980年，是英国领先的定制肽和抗体工具的独立生产商，为全球制药，生命科学和学术领域的研究人员提供产品。其产品的主要应用是定制的实验室规模试剂，用于早期药物发现中的主要研究证据，并支持临床研究中的生物标志物计划。该公司生产各种口味的肽，从简单到修饰，稳定和放射性标记，再到从毫克到克到最高纯度的染料标记。此外，CRB是产生高度特异性和靶向抗体（多克隆抗体和单克隆抗体）的公认专家。荧光染料标记也是重点。自1994年以来，该公司于2016年启动了其首个目录业务DISCOVERY®。
