发现抗体/抗KV1.3(KCNA3)(细胞外)-PE抗体/2 x 50µl/APC-101-PE-2 x 50µl

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¥14740.00
货号:APC-101-PE-2x50µl
浏览量:116
品牌:Discovery
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商品描述
Cat#:APC-101-PE
AlternativeNamePotassiumvoltage-gatedchannelsubfamilyAmember3
  • LyophilizedPowder
  • Type:Polyclonal
    Host:Rabbit
    Reactivity:h,m,r
      • PeptideKDYPASTSQDSFEA(C),correspondingtoaminoacidresidues263-276ofhumanKV1.3(AccessionP22001).1stextracellularloop.
        Anti-KV1.3 (KCNA3) (extracellular)-PE Antibody
    Accession(Uniprot)NumberP22001
    GeneID3738
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyRat,mouse–12/14aminoacidresiduesidentical.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    IsotypeRabbitIgG.
    LabelR-Phycoerythrin(R-PE).
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution50µldoubledistilledwater(DDW).
    Antibodyconcentrationafterreconstitution1mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).
    StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#APC-101),anddirectflowcytometry(labeledantibody).
    Applications:fc
    Mayalsoworkin:ic*,lci*
      • Anti-KV1.3 (KCNA3) (extracellular)-PE Antibody
        CellsurfacedetectionofKV1.3inhumanJurkatT-cellleukemiacells:
        ___ Cells.
        ___ Cells+rabbitIgGisotypecontrol-PE.
        ___ Cells+ Anti-KV1.3(KCNA3)(extracellular)-PEAntibody(#APC-101-PE),(5µg).
        Anti-KV1.3 (KCNA3) (extracellular)-PE Antibody
        CellsurfacedetectionofKV1.3inmouseTK-1T-celllymphomacells:
        ___ Cells.
        ___ Cells+rabbitIgGisotypecontrol-PE.
        ___ Cells+ Anti-KV1.3(KCNA3)(extracellular)-PEAntibody(#APC-101-PE),(5µg).
    References
    • 1.Chandy,K.G. etal. (2001) Toxicon 39, 1269.
    • 2.Koo,G.C. etal. (1997) J.Immunol. 158, 5120.
    • 3.Grissmer,S. etal. (1994) Mol.Pharmacol. 45, 1227.
    • 4.Garcia-Calvo,M. etal. (1993) J.Biol.Chem. 268, 18866.
    • 5.Garcia,M.L. etal. (1994) Biochemistry 33, 6834.
    • 6.Koschak,A. etal. (1998) J.Biol.Chem. 273, 2639.
    • 7.Kalman,K. etal. (1998) J.Biol.Chem. 273, 32697.
      • KV1.3 belongstothe Shaker familyof voltage-dependentK+ channels.Thechannel,encodedby KCNA3,iswidelyexpressedinthebrain,lungandosteoclastsandinseveralcell populationsofhematopoieticorigin.TheprominenceofKV1.3channelsinthesecells(particularlyinTlymphocytes)directedmuchresearch attention.ItwasfoundthatKV1.3isthemainchannelresponsIBLeformaintainingtherestingpotentialinquiescentcellsandregulatingthe Ca2+ signalingthatisindispensablefornormalTlymphocyteactivation.1,2 BasedonthecentralroleofKV1.3inregulatingtheinitiationofanimmuneresponse, thechannelhasbeenrecognizedasapotentialtargetforimmunosuppressantdrugs.1

        KV1.3channelsarepotentlyinhibitedbyseveralvenomouspeptidetoxinsamongthem CharyBDotoxin (2.6nM), Noxiustoxin (1nM), Kaliotoxin (0.65nM), Margatoxin (0.05nM), Agitoxin-1 (1.7nM), Agitoxin-2 (0.004nM), Hongotoxin-1 (0.09nM)and Stichodactylatoxin (0.01nM).3-7

        ThecentralroleofKV1.3inimmunesystemcellscreatedarealneedforaspecific antibodythatwouldbeabletoworkinflowcytometryapplications.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IF-Immunofluorescence,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    Lastupdate:27/11/2019

    Anti-KV1.3(KCNA3)(extracellular)Antibody (#APC-101)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanprotein.Theantibody canbeusedinwesternblot,immunohistochemistry,livecellimagingandindirectflowcytometryapplications.IthasbeendesignedtorecognizeKV1.3potassiumchannelfromhuman,mouseandratsamples.

    Anti-KV1.3(KCNA3)(extracellular)-PEAntibody (#APC-101-PE)isdirectlyconjugatedtoPhycoerythrin(PE).Thislabeledantibody canbeusedinimmunofluorescentapplicationssuchasdirectflowcytometryandlivecellimaging.

    Forresearchpurposesonly,notforhumanuse
    Discovery®抗体是Cambridge Research Biochemicals的目录抗体业务。 CRB是全球第二大最古老的肽公司,也是抗体研究市场的先驱,目前正以Discovery®Antibodies的名义建立一系列新验证的靶标抗体。 Discovery®抗体将专注于研究用抗体(早期药物“发现”),并将补充我们非常成功且长期存在的定制抗体业务(www.crbdiscovery.com)。我们已经以新颖,复杂,稀有和翻译后修饰的特异性抗体而闻名,这些受追捧的靶标将出现在我们的Discovery®Antibody目录范围内。  我们于2017年2月,在发现肽之后一年,并且在詹姆斯·库克船长(他当时最伟大的探险家)于1776年从英国出发,乘坐“ HMS发现”号开始租用新水域和“发现全球各地的新土地。因此,“发现”这个名称似乎适合东北制造商–詹姆斯·库克的故乡。 在1700年代后期对全球新发现土地的追求与医学研究的新“发现”以及2000年人类基因组图谱的发现之间,还有其他相似之处。  有关更多详细信息,请参见有关Discovery®抗体发布的新闻稿。   Cambridge Research Biochemicals  (www.crbdiscovery.com)成立于1980年,是英国领先的定制肽和抗体工具的独立生产商,为全球制药,生命科学和学术领域的研究人员提供产品。其产品的主要应用是定制的实验室规模试剂,用于早期药物发现中的主要研究证据,并支持临床研究中的生物标志物计划。该公司生产各种口味的肽,从简单到修饰,稳定和放射性标记,再到从毫克到克到最高纯度的染料标记。此外,CRB是产生高度特异性和靶向抗体(多克隆抗体和单克隆抗体)的公认专家。荧光染料标记也是重点。自1994年以来,该公司于2016年启动了其首个目录业务DISCOVERY®。
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