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LyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.
- PeptideKDYPASTSQDSFEA(C),correspondingtoaminoacidresidues263-276ofhumanKV1.3(AccessionP22001).1stextracellularloop.

CellsurfacedetectionofKV1.3inhumanJurkatT-cellleukemiacells:___ Cells.
___ Cells+rabbitIgGisotypecontrol-PE.
___ Cells+ Anti-KV1.3(KCNA3)(extracellular)-PEAntibody(#APC-101-PE),(5µg).
CellsurfacedetectionofKV1.3inmouseTK-1T-celllymphomacells:___ Cells.
___ Cells+rabbitIgGisotypecontrol-PE.
___ Cells+ Anti-KV1.3(KCNA3)(extracellular)-PEAntibody(#APC-101-PE),(5µg).
- 1.Chandy,K.G. etal. (2001) Toxicon 39, 1269.
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- 3.Grissmer,S. etal. (1994) Mol.Pharmacol. 45, 1227.
- 4.Garcia-Calvo,M. etal. (1993) J.Biol.Chem. 268, 18866.
- 5.Garcia,M.L. etal. (1994) Biochemistry 33, 6834.
- 6.Koschak,A. etal. (1998) J.Biol.Chem. 273, 2639.
- 7.Kalman,K. etal. (1998) J.Biol.Chem. 273, 32697.
KV1.3 belongstothe Shaker familyof voltage-dependentK+ channels.Thechannel,encodedby KCNA3,iswidelyexpressedinthebrain,lungandosteoclastsandinseveralcell populationsofhematopoieticorigin.TheprominenceofKV1.3channelsinthesecells(particularlyinTlymphocytes)directedmuchresearch attention.ItwasfoundthatKV1.3isthemainchannelresponsIBLeformaintainingtherestingpotentialinquiescentcellsandregulatingthe Ca2+ signalingthatisindispensablefornormalTlymphocyteactivation.1,2 BasedonthecentralroleofKV1.3inregulatingtheinitiationofanimmuneresponse, thechannelhasbeenrecognizedasapotentialtargetforimmunosuppressantdrugs.1
KV1.3channelsarepotentlyinhibitedbyseveralvenomouspeptidetoxinsamongthem CharyBDotoxin (2.6nM), Noxiustoxin (1nM), Kaliotoxin (0.65nM), Margatoxin (0.05nM), Agitoxin-1 (1.7nM), Agitoxin-2 (0.004nM), Hongotoxin-1 (0.09nM)and Stichodactylatoxin (0.01nM).3-7
ThecentralroleofKV1.3inimmunesystemcellscreatedarealneedforaspecific antibodythatwouldbeabletoworkinflowcytometryapplications.
Anti-KV1.3(KCNA3)(extracellular)Antibody (#APC-101)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanprotein.Theantibody canbeusedinwesternblot,immunohistochemistry,livecellimagingandindirectflowcytometryapplications.IthasbeendesignedtorecognizeKV1.3potassiumchannelfromhuman,mouseandratsamples.
Anti-KV1.3(KCNA3)(extracellular)-PEAntibody (#APC-101-PE)isdirectlyconjugatedtoPhycoerythrin(PE).Thislabeledantibody canbeusedinimmunofluorescentapplicationssuchasdirectflowcytometryandlivecellimaging.


