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- Liao,C.F. etal. (1989) J.Biol.Chem. 264, 7328.
- Lazareno,S. etal. (1990) Br.J.Pharmacol. 109, 1120.
- Hernandez,M. etal. (1993) Br.J.Pharmacol. 110, 1413.
AlomoneLabsTropicamideinhibitscarbachol’seffectonM1mAChRexpressedinC6cells.CellswereloadedwithFluo-3AMandchangesinintracellularCa2+ weredetectedviachangesinFluo-3emission.A.Normalizedfluorescencebeforeandafterapplication(at20seconds,seearrow)of10μMCarbacholfollowinga20minincubationwith0-100μM Tropicamide (#T-120)(asindicated).B.Inhibition(aspercentofcontrol)of10µMCarbacholevokedCa2+ rise,plottedagainstTropicamideconcentrations.
- 1.Bonner,T.I. etal. (1989) Trends.Neurosci. 12, 148.
- 2.Felder,C.C. etal. (1995) FASEB.J. 9, 619.
- 3.Bonner,T.I. etal. (1987) Science 237, 527.
- 4.Peralta,E.G. etal. (1987) EMBOJ. 6, 3923.
- 5.Bonner,T.I. etal. (1988) Neuron 1, 403.
- 6.Liao,C.F. etal. (1989) J.Biol.Chem. 264, 7328.
- 7.Liao,C.F. etal. (1989) J.Biol.Chem. 264, 7328.
- 8.Lazareno,S. etal. (1990) Br.J.Pharmacol. 109, 1120.
- 9.Hernandez,M. etal. (1993) Br.J.Pharmacol. 110, 1413.
- 10.Manny,R.E. etal. (2001) Invest.Ophtalmol.Vis.Sci. 42, 1728.
Muscarinicacetylcholinereceptors(mAChR)regulateanumberofimportantbasicphysiologicfunctionsincludingheartrate,motorandsensorycontrolandmorecomplexbehaviorsincludingarousal,memory,andlearning.LossofmuscarinicreceptornumberorfunctionhasbeenimplicatedintheetiologyofseveralneurologicaldisordersincludingAlzheimer"sdementia,Down"ssyndromeandParkinson"sdisease.
Fivesubtypesofmuscarinicreceptors(m1-m5)havebeenidentifiedbymolecularcloning1andmuchhasbeenlearnedabouttheirdistribution,pharmacology,andstructure2-6.
Tropicamideisamuscarinicreceptorantagonist,whichshowsa3foldselectivityform47-8.ItseffecthasapIC50of~7.5forporcineintravesicaluretercontraction9.
Tropicamideproducesshortactingmydriasis(dilationofthepupil)andcycloplegia(paralysisoftheciliarymuscle)whenappliedaseyedrops.Itisalsoaneffectivecycloplegicagentinmyopicchildren10.
Tropicamide(#T-120) isahighlypure,synthetic,andbiologicallyactivecompound.



