发现抗体/抗P2Y2受体(细胞外)-FITC抗体/10 x 50µl/APR-102-F-10 x 50µl

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¥35940.00
货号:APR-102-F-10x50µl
浏览量:127
品牌:Discovery
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商品描述
Cat#:APR-102-F
AlternativeNameP2Ypurinoceptor2,P2RY2,P2U1
  • LyophilizedPowder
  • Type:Polyclonal
    Host:Rabbit
    Reactivity:h,m,r
      • Peptide(CD)TINGTWEGDELGYK,correspondingtoaminoacidresidues11-24ofratP2RY2(AccessionP41232).Extracellular,C-terminus.
        Anti-P2Y2 Receptor (extracellular)-FITC Antibody
    Accession(Uniprot)NumberP41232
    GeneID29597
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyRat,mouse–14/15aminoacidresiduesidentical;human–13/15aminoacidresiduesidentical.
    RRIDAB_2756770.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    IsotypeRabbitIgG.
    LabelFluoresceinisothiocyanate(FITC).
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution50µldoubledistilledwater(DDW).
    Antibodyconcentrationafterreconstitution1mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).
    StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#APR-102),anddirectflowcytometry(labeledantibody).
    Applications:fc
    Mayalsoworkin:ic*,lci*
      • Anti-P2Y2 Receptor (extracellular)-FITC Antibody
        CellsurfacedetectionofP2RY2inliveintactmouseJ774macrophagecells:
        ___ Cells.
        ___ Cells+rabbitIgGisotypecontrol-FITC.
        ___ Cells+Anti-P2Y2Receptor(extracellular)-FITCAntibody(#APR-102-F),(2.5µg).
        Anti-P2Y2 Receptor (extracellular)-FITC Antibody
        CellsurfacedetectionofP2RY2inliveintacthumanTHP-1monocyticleukemiacells:
        ___ Cells.
        ___ Cells+rabbitIgGisotypecontrol-FITC.
        ___ Cells+Anti-P2Y2Receptor(extracellular)-FITCAntibody(#APR-102-F),(5µg).
    References
    • 1.Wang,L.etal.(2002)J.Cardiovas.Pharmacol.40,841.
    • 2.Queiroz,G.etal.(2003)J.Pharmacol.Exp.Ther.307,809.
    • 3.Ralevic,V.andBurnstock,G.(1998)Pharmacol.Rev.50,413.
    • 4.Abbracchio,M.P.etal.(2003)TrendsPharmacol.Sci.24,52.
    • 5.DiVirgilio,F.etal.(2001)Blood97,587.
    • 6.Yerxa,B.R.etal.(2002)J.Pharmacol.Exp.Ther.302,871.
      • P2Yreceptors belongtotheG-proteincoupledreceptorsuperfamily.P2Yreceptorsmediatetheactionsoftheextracellularnucleotides(ATP,ADP,UTPandUDP)andregulateseveralphysiologicalresponses,amongthem,cardiacfunction,plateletaggregationandSMCproliferation1.

        ThemammalianP2Yfamilycurrentlyincludeseightfunctionalreceptors; P2Y1,P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13,andtheUDP-glucosereceptor,nowrenamed P2Y142-4.

        TheP2Y2receptorisabundantlyexpressedintheapicalmembranesofseveralepithelialtissuesincludingairway5.IthasbecomealeADIngtargetforthetreatmentofcysticfibrosis.ActivationofP2RY2increasesCl- effluxandmucociliaryclearance,effectsthatmightrelievesymptomsofcysticfibrosis.P2Y2agoNISTtherapyistestedinanattempttobypassthedysfunctioningCFTRchannel.SeveralP2Y2receptoragonists, especiallyINS37217,beingevaluatedaspossIBLetargetsforthetreatmentofcysticfibrosis6.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IF-Immunofluorescence,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    Lastupdate:25/12/2019

    Anti-P2Y2Receptor(extracellular)Antibody (#APR-102)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblot,indirectlivecellflowcytometry,andimmunohistochemistryapplications.IthasbeendesignedtorecognizeP2RY2fromrat,mouse,andhumansamples.

    Anti-P2Y2Receptor(extracellular)FITCAntibody (#APR-102-F)isdirectlyconjugatedtofluoresceinisothiocyanate(FITC).Thislabeledantibody canbeusedinimmunofluorescentapplicationssuchasdirectflowcytometryusinglivecells.

    Forresearchpurposesonly,notforhumanuse
    Discovery®抗体是Cambridge Research Biochemicals的目录抗体业务。 CRB是全球第二大最古老的肽公司,也是抗体研究市场的先驱,目前正以Discovery®Antibodies的名义建立一系列新验证的靶标抗体。 Discovery®抗体将专注于研究用抗体(早期药物“发现”),并将补充我们非常成功且长期存在的定制抗体业务(www.crbdiscovery.com)。我们已经以新颖,复杂,稀有和翻译后修饰的特异性抗体而闻名,这些受追捧的靶标将出现在我们的Discovery®Antibody目录范围内。  我们于2017年2月,在发现肽之后一年,并且在詹姆斯·库克船长(他当时最伟大的探险家)于1776年从英国出发,乘坐“ HMS发现”号开始租用新水域和“发现全球各地的新土地。因此,“发现”这个名称似乎适合东北制造商–詹姆斯·库克的故乡。 在1700年代后期对全球新发现土地的追求与医学研究的新“发现”以及2000年人类基因组图谱的发现之间,还有其他相似之处。  有关更多详细信息,请参见有关Discovery®抗体发布的新闻稿。   Cambridge Research Biochemicals  (www.crbdiscovery.com)成立于1980年,是英国领先的定制肽和抗体工具的独立生产商,为全球制药,生命科学和学术领域的研究人员提供产品。其产品的主要应用是定制的实验室规模试剂,用于早期药物发现中的主要研究证据,并支持临床研究中的生物标志物计划。该公司生产各种口味的肽,从简单到修饰,稳定和放射性标记,再到从毫克到克到最高纯度的染料标记。此外,CRB是产生高度特异性和靶向抗体(多克隆抗体和单克隆抗体)的公认专家。荧光染料标记也是重点。自1994年以来,该公司于2016年启动了其首个目录业务DISCOVERY®。
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