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- Berridge,M.J. (1993) Nature 361,315.
- Scharff,O. etal.(1993) Physiol. Rev. 73,547.
- Orrenius,S. etal. (2003) Nat.Rev.Mol.CellBiol. 4,552.
- Hajnoczky,G. etal. (2003) Biochem.Biophys.Res.Commun. 304,445.
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AlomoneLabsA23187inducesapoptosisinJurkatcells.2µM A23187 (#A-600)orvehiclewasaddedtotheconfluentcellculturefor6hours.ApoptosiswasprobedbythedetectionofcleavedCaspase3usingCaspase3specificantibodiesinwesternblotanalysisofcelllysates.
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A23187isamobile-carrierCa2+ionophore(allowsCa2+ionstocrosscellmembranes)originallyisolatedasanantibioticfromStreptomyceschartreusensis.ItisusedtoincreaseintracellularCa2+levelsandforinvitrostudiesrelatedtointracellularCa2+-mediatedevents.A23187cancausecellactivation,differentiation,orproliferationandthusmimicscellularprocessesobservedinresponsetocytokinesandgrowthfactors.1,2
Furthermore,A23187induces,inaconcentration-andtime-dependentmanner,Ca2+-triggeredcellapoptosis.3,4Thispropertywasexploitedtoclarifyapoptoticeventsindifferentcelllinessuchasmouselymphomacellline(S49),5,6histiocyticlymphomaU937,7humanmyeloidleukemiacells(HL-60),8culturedstriatalneurons,9renaltubularcells,10andC6gliomacells.11
A23187(#A-600) isahighlypure,natural,andbiologicallyactivecompound.



