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LyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.
- PeptideCKQGFLSSNGQNHFK,correspondingtoaminoacidresidues58-72ofhumanEMR1(Accession Q14246).Extracellular,N-terminus.

CellsurfacedetectionofEMR1inliveintactmouseJ774macrophagecells:___ Cells.
___ Cells+rabbitIgGisotypecontrol-FITC.
___ Cells+Anti-EMR1(ADGRE1)(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).
CellsurfacedetectionofEMR1inliveintacthumanTHP-1monocyticleukemiacells:___ Cells.
___ Cells+rabbitIgGisotypecontrol-FITC.
___ Cells+Anti-EMR1(ADGRE1)(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).
- 1.Legrand,F.etal.(2014)J.AllergyClin.Immunol.133,1439.
- 2.McKnight,A.J.andGordon,S.(1998)J.Leukoc.Biol.63,271.
- 3.Araç,D.etal.(2012)EMBOJ.31,1364.
Humanepidermalgrowthfactor(EGF)–likemodulecontainingmucin-likehormonereceptor1(EMR1)isasurfacereceptorwithunknownfunctionthatbelongstotheadhesionfamilyoftheGprotein-coupledreceptors(GPCR).ThisreceptorisencodedbytheADGRE1andlikeallGPCRs,containsseventransmembranedomains,anintracellularC-terminusandanextracellularN-terminus.TheNH2domainhassixEGF-likedomains.
AdhesionGPCRssharealongN-terminalextracellularsequencescomprisingmultipledomains,separatedfromthetransmembranesegmentsbyaserine/threonine-richdomain,afeaturereminiscentofmucin-like,single-span,integralmembraneglycoproteinswithadhesiveproperties1,2.
EMR1proteinisahomologofF4/80,amurineproteinthatiswidelybeingusedasaMarkerofmurinemacrophagepopulations1.
HumanEMR1isexclusivelyexpressedonmatureeosinophilsinthebloodandbonemarrow andinnasalpolyps.Thus,EMR1is consideredtobeapotentialtherapeutictargetforthetreatmentofeosinophilicdisorders1,3.
Anti-EMR1(ADGRE1)(extracellular)Antibody (#AER-051)isahighlyspecificantibodydirectedagainstanepitopeofthehumanprotein.Theantibodycanbeusedinwesternblotandlivecellimagingapplications.Itrecognizesanextracellularepitopeandisthusidealfordetectingthereceptorinlivingcells.IthasbeendesignedtorecognizeEMR1fromhuman,mouse,andratsamples.
Anti-EMR1(ADGRE1)(extracellular)–FITCAntibody (#AER-051-F)is directlyconjugatedtofluoresceinisothiocyanate(FITC). The antibodycanbeusedinimmunofluorescentapplicationssuchasdirectlivecellflowcytometry.


