发现抗体/豚鼠抗KCNN2(KCa2.2,SK2)抗体/25µl/AGP-045-25µl

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¥4940.00
货号:AGP-045-25µl
浏览量:127
品牌:Discovery
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商品描述
Cat#:APC-002
AlternativeNamePotassiumvoltage-gatedchannelsubfamilyAmember3
  • LyophilizedPowder
  • AntigenIncl.
  • Type:Polyclonal
    Host:Rabbit
    Reactivity:h,m,r
      • GSTfusionproteinwiththesequenceTLSKSEYMVIEEGGMNHSAFPQTPFKTGNSTATCTTNNNPNSCVNIKKIFTDV,correspondingtoaminoacidresidues523-575ofhumanKV1.3(Accession P22001).Intracellular,C-terminus.
        Anti-KV1.3 (KCNA3) Antibody
    Accession(Uniprot)NumberP22001
    GeneID3738
    PeptideconfirmationConfirmedbyDNAsequenceandSDS-PAGE.
    HomologyRat,rabbit,mouse-identical.
    RRIDAB_2040151.
    PurityTheserumwasdepletedofanti-GSTantibodiesbyaffinitychromatographyonimmobilizedGSTandthentheIgGfractionwaspurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,5%sucrose,0.025%NaN3.
    IsotypeRabbitIgG.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
    Antibodyconcentrationafterreconstitution0.6mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
    NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Negativecontrolantigenreconstitution100μlPBS.
    Negativecontrolantigenstorageafterreconstitution-20°C.
    PreadsorptionControl3µgfusionproteinper1µgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis.
    Applications:ic,if,ih,ip,wb
    Mayalsoworkin:ifc*
      • Anti-KV1.3 (KCNA3) Antibody
        Westernblotanalysis ofratbrainmembranes:
        1. Anti-KV1.3 (KCNA3) Antibody(#APC-002),(1:200).
        2.Anti-KV1.3(KCNA3)Antibody,preincubatedwiththenegativecontrolantigen.
        HumanCD3+cells(Szigligeti,P. etal. (2006) J.Physiol. 573, 357.).
      • TransfectedHEK-293celllysate(Vicente,R. etal. (2006) J.Biol.Chem. 281, 37675.).
      • Ratbrainsections.
      • RatneuralProgenitorcells(NPCs)(1:200)(Liebau,S. etal. (2006) J.NeuRochem99, 426.).
    References
    • 1.Chandy,K.G.etal.(2001)Toxicon39,1269.
    • 2.Koo,G.C.etal.(1997)J.Immunol.158,5120.
    • 3.Grissmer,S.etal.(1994)Mol.Pharmacol.45,1227.
    • 4.Garcia-Calvo,M.etal.(1993)J.Biol.Chem.268,18866.
    • 5.Garcia,M.L.etal.(1994)Biochemistry33,6834.
    • 6.Koschak,A.etal.(1998)J.Biol.Chem.273,2639.
    • 7.Kalman,K.etal.(1998)J.Biol.Chem.273,32697.
      • KV1.3belongstothe Shaker familyof voltage-dependentK+ channels. Thechannel,encodedby KCNA3,iswidelyexpressedinthebrain,lungandosteoclastsandinseveralcell populationsofhematopoieticorigin.TheprominenceofKV1.3channelsinthesecells(particularlyinTlymphocytes)directedmuchresearch attention.ItwasfoundthatKV1.3isthemainchannelresponsIBLeformaintainingtherestingpotentialinquiescentcellsandregulatingthe Ca2+ signalingthatisindispensablefornormalTlymphocyteactivation.1,2 BasedonthecentralroleofKV1.3inregulatingtheinitiationofanimmuneresponse, thechannelhasbeenrecognizedasapotentialtargetforimmunosuppressantdrugs.1

        KV1.3channelsarepotentlyinhibitedbyseveralvenomouspeptidetoxinsamongthem CharyBDotoxin (2.6nM), Noxiustoxin (1nM), Kaliotoxin (0.65nM), Margatoxin (0.05nM), Agitoxin-1 (1.7nM), Agitoxin-2 (0.004nM), Hongotoxin-1 (0.09nM) and Stichodactylatoxin (0.01nM).3-7

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IF-Immunofluorescence,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    receivea25µlfreetrialsample!
    Lastupdate:08/11/2019

    AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanepitopeofhumanKV1.3.Anti-KV1.3(KCNA3)Antibody(#APC-002)canbeusedinwesternblot,immunoprecipitation,immunocytochemistry,andimmunohistochemistryapplications.IthasbeendesignedtorecognizeKV1.3potassiumchannelfromhuman,rat,andmousesamples.

    Forresearchpurposesonly,notforhumanuse
    Discovery®抗体是Cambridge Research Biochemicals的目录抗体业务。 CRB是全球第二大最古老的肽公司,也是抗体研究市场的先驱,目前正以Discovery®Antibodies的名义建立一系列新验证的靶标抗体。 Discovery®抗体将专注于研究用抗体(早期药物“发现”),并将补充我们非常成功且长期存在的定制抗体业务(www.crbdiscovery.com)。我们已经以新颖,复杂,稀有和翻译后修饰的特异性抗体而闻名,这些受追捧的靶标将出现在我们的Discovery®Antibody目录范围内。  我们于2017年2月,在发现肽之后一年,并且在詹姆斯·库克船长(他当时最伟大的探险家)于1776年从英国出发,乘坐“ HMS发现”号开始租用新水域和“发现全球各地的新土地。因此,“发现”这个名称似乎适合东北制造商–詹姆斯·库克的故乡。 在1700年代后期对全球新发现土地的追求与医学研究的新“发现”以及2000年人类基因组图谱的发现之间,还有其他相似之处。  有关更多详细信息,请参见有关Discovery®抗体发布的新闻稿。   Cambridge Research Biochemicals  (www.crbdiscovery.com)成立于1980年,是英国领先的定制肽和抗体工具的独立生产商,为全球制药,生命科学和学术领域的研究人员提供产品。其产品的主要应用是定制的实验室规模试剂,用于早期药物发现中的主要研究证据,并支持临床研究中的生物标志物计划。该公司生产各种口味的肽,从简单到修饰,稳定和放射性标记,再到从毫克到克到最高纯度的染料标记。此外,CRB是产生高度特异性和靶向抗体(多克隆抗体和单克隆抗体)的公认专家。荧光染料标记也是重点。自1994年以来,该公司于2016年启动了其首个目录业务DISCOVERY®。
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