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LyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!
- GSTfusionproteinwiththesequenceTLSKSEYMVIEEGGMNHSAFPQTPFKTGNSTATCTTNNNPNSCVNIKKIFTDV,correspondingtoaminoacidresidues523-575ofhumanKV1.3(Accession P22001).Intracellular,C-terminus.

Westernblotanalysis ofratbrainmembranes:1. Anti-KV1.3 (KCNA3) Antibody(#APC-002),(1:200).
2.Anti-KV1.3(KCNA3)Antibody,preincubatedwiththenegativecontrolantigen.HumanCD3+cells(Szigligeti,P. etal. (2006) J.Physiol. 573, 357.).
- TransfectedHEK-293celllysate(Vicente,R. etal. (2006) J.Biol.Chem. 281, 37675.).
- Ratbrainsections.
- 1.Chandy,K.G.etal.(2001)Toxicon39,1269.
- 2.Koo,G.C.etal.(1997)J.Immunol.158,5120.
- 3.Grissmer,S.etal.(1994)Mol.Pharmacol.45,1227.
- 4.Garcia-Calvo,M.etal.(1993)J.Biol.Chem.268,18866.
- 5.Garcia,M.L.etal.(1994)Biochemistry33,6834.
- 6.Koschak,A.etal.(1998)J.Biol.Chem.273,2639.
- 7.Kalman,K.etal.(1998)J.Biol.Chem.273,32697.
KV1.3belongstothe Shaker familyof voltage-dependentK+ channels. Thechannel,encodedby KCNA3,iswidelyexpressedinthebrain,lungandosteoclastsandinseveralcell populationsofhematopoieticorigin.TheprominenceofKV1.3channelsinthesecells(particularlyinTlymphocytes)directedmuchresearch attention.ItwasfoundthatKV1.3isthemainchannelresponsIBLeformaintainingtherestingpotentialinquiescentcellsandregulatingthe Ca2+ signalingthatisindispensablefornormalTlymphocyteactivation.1,2 BasedonthecentralroleofKV1.3inregulatingtheinitiationofanimmuneresponse, thechannelhasbeenrecognizedasapotentialtargetforimmunosuppressantdrugs.1
KV1.3channelsarepotentlyinhibitedbyseveralvenomouspeptidetoxinsamongthem CharyBDotoxin (2.6nM), Noxiustoxin (1nM), Kaliotoxin (0.65nM), Margatoxin (0.05nM), Agitoxin-1 (1.7nM), Agitoxin-2 (0.004nM), Hongotoxin-1 (0.09nM) and Stichodactylatoxin (0.01nM).3-7
AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanepitopeofhumanKV1.3.Anti-KV1.3(KCNA3)Antibody(#APC-002)canbeusedinwesternblot,immunoprecipitation,immunocytochemistry,andimmunohistochemistryapplications.IthasbeendesignedtorecognizeKV1.3potassiumchannelfromhuman,rat,andmousesamples.


