1-EBIO is a potent K_Ca2/3 channel activator that activates the channels by increasing the Ca²⁺ sensitivity by stabilizing the interaction between calmodulin and the channels^1,2, thus shifting the activation curve of the channels to the left. 1-EBIO activates K_Ca3.1 with an EC₅₀ value of around 30 μM and all three K_Ca2 channels with EC₅₀ value around 300 μM².

The Ca²⁺-activated K⁺ channel, K_Ca3.1, plays a major role in the controlled expression of essential genes that are responsible for several physiological processes including- membrane potential, cell volume proliferation, migration and production of inflammatory mediators by participating in the regulation of calcium entry. A highly regulated and sustained increase in cytosolic Ca²⁺ is critical in these processes making K_Ca3.1 a potential target for therapy of several disease^1,3.