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蚂蚁淘在线 / 品牌中心 / Discovery / Discovery Antibodies/Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO-488 Antibody/50 µl/AAR-012-AG-50 µl

Discovery Antibodies/Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO-488 Antibody/50 µl/AAR-012-AG-50 µl

价格
￥10540.00
货号：AAR-012-AG-50µl
浏览量：127
品牌：Discovery
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商品描述
Cat#:AAR-012-AG
AlternativeNameAT2 Receptor,AT2R,AGTR2,MRX88
  • LyophilizedPowder
  • AntigenIncl.
    • Type:Polyclonal
    Host:Rabbit
    Reactivity:m,r
      • PeptideDNLNATGTNESAFNC,correspondingtoaminoacidresidues21-35ofratAT2receptor(AccessionP35351).Extracellular,N-terminus.
        Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO-488 Antibody
    Accession(Uniprot)NumberP35351
    GeneID24182
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyMouse-identical.
    RRIDAB_2039724.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    IsotypeRabbitIgG.
    SpecificityWillnotrecognizehumanAT2Receptor.
    LabelATTO-488.Maximumabsorption501nm;maximumfluorescence523nm.Thefluorescenceisexcitedmostefficientlyinthe480–515nmrange.Thislabelisanalogoustothewellknowndyefluoresceinisothiocyanate(FITC)andcanbeusedwithfilterstypicallyusedtodetectFITC.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution50µldoubledistilledwater(DDW).
    Antibodyconcentrationafterreconstitution1mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).
    NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).
    Negativecontrolantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1μgpeptideper1μgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AAR-012),andimmunohistochemistry(labeledantibody).
    Applications:ic,if,ih,lci
      • Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO-488 Antibody
        ExpressionofAngiotensinIIreceptortype-2inratbrain
        Immunohistochemicalstainingofratfrozenfreefloatingbrain sectionsusingAnti-AngiotensinII ReceptorType-2(extracellular)-ATTO-488Antibody(#AAR-012-AG),(1:20).AT2receptor(green)wasdetectedinneuronsinthevicinityofthehypothalamicparaventricularnucleus(PVN).Insomeneurons(thickarrow),axonalprocesseswithvaricositieswereobserved(thinarrow).NucleiwerevisualizedwithDAPIcounterstain(blue).
        Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO-488 Antibody
        Immuno-colocalizationofAT2 receptorandVGLUT2inratsupraoptichypothalamicnucleus
        Immunohistochemicalstainingofperfusion-fixedfrozenratbrainsectionsusing Anti-AngiotensinII ReceptorType-2(extracellular)-ATTO-488Antibody(#AAR-012-AG),(1:60)and Anti-VGLUT2-ATTO-594Antibody (#AGC-036-AR),(1:60).A.AT2 receptorstaining(green).B.VGLUT2staining(red).C.Mergeofthetwoimagesshowsagenerallackofco-localizationofAT2RandVGLUT2inthispartofthehypothalamus(arrow).CellnucleiarestainedwithDAPI(blue).
      • Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO-488 Antibody
        ExpressionofAngiotensinIIreceptortype-2in mouse3T3-L1cells
        Cellsurfacedetectionof AngiotensinIIreceptortype-2inintactlivemouse3T3-L1cellswithAnti-AngiotensinIIReceptorType-2(extracellular)-ATTO-488 Antibody(#AAR-012-AG),(1:50),(green).Liveviewofthesamefieldwassuperimposedtothefluorescentone.
    References
    • 1.Mukoyama,M.etal.(1993)J.Biol.Chem.268,24539.
    • 2.deGasparo,M.etal.(2000)Pharmacol.Rev.52,415.
    • 3.Carey,R.M.andSiragy,H.M.(2003)Endocr.Rev.24,261.

      • AngiotensinIIreceptortype2orAT2 isoneofthereceptorsthatbindtheoctapeptidehormoneAngiotensinII(AngII).
         
        AngIIisthepeptidehormonethatgeneratesmostoftheknowneffectsoftherenin-angiotensinsystem(RAS).AngIIisgeneratedfromtheangiotensinogenproteinbytheactionsofrenin,angiotensinconvertingenzyme(ACE)andotherpeptidases.AngIIhasacentralroleincardiovascularhomeostasisbyregulatingvasoconstriction,renalNa+ andwaterreadsorption.Inaddition,AngIIinducescellgrowthandproliferationandhaspro-inflammatoryeffects.
         
        MostofthephysiologicalactionsofAngIIaremediatedbyAT1 amemberofthe7-transmembranedomain,Gprotein-coupledreceptor(GPCR)superfamily.AT2 isalsoaGPCR,and islargelybelievedtohavecounter-regulatoryrolestotheonesexertedthroughtheAT1 receptor.Hence,bindingofAngIItotheAT2 receptorwillresultingrowtharrestandapoptosis,vasodilatationandhypotension.
         
        AlthoughtheAT2 receptorisstructurallyamemberoftheGPCRsuperfamily,thesignalingmechanismselicitedfollowingAT2 receptoractivationarenotfullyclarified.RecentevidenceindicatesthattheAT2 receptorsignalsthroughtheGiα2 andtheGiα3 proteinsandthroughtheactivationofphosphotyrosinephosphatasessuchas SHP-1.
         
        AT2 receptordistributionismorerestrictedthanthatofthe AT1 receptor.ThehighestexpressionlevelsofAT2 havebeenfoundinthefetus,whichisfollowedbyamarkeddecreaseshortlyafterbirth.Nevertheless,significantAT2 receptorexpressioncanbedetectedintheheart,kidney,vascularendothelialcellsandbrain,amongothers.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,
    IF-Immunofluorescence,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    LyophilizedPowderNegativeControlAntigenIncluded
    Image&Title:

    Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO-488 Antibody
    Immuno-colocalizationofMelatoninReceptorType1BandAT2 Receptorinratbrain    Immunohistochemicalstainingofperfusion-fixedfrozenbrainsectionsusing Anti-MelatoninReceptor1B(MTNR1B)Antibody (#AMR-032),(1:600)and Anti-AngiotensinIIReceptorType-2(extracellular)-ATTO-488 Antibody(#AAR-012-AG),(1:100).A.MTNR1Bstaining(red)(arrows).B.ThesamesectionlabeledforAT2receptor(green).C.Mergeofthetwoimagessuggestsconsiderableco-localizationintheparaventricularnucleus(arrows).Fororientation,notelocalizationwithrespectto3rd ventricle(3rd V).

    Lastupdate:10/11/2019

    Anti-AngiotensinIIReceptorType-2(extracellular)Antibody (#AAR-012)isahighlyspecificantibodydirectedagainstanextracellularepitopeoftheratprotein.The antibodycanbeusedinwesternblot,immunocytochemistryandimmunohistochemistryapplications.IthasbeendesignedtorecognizeAT2receptorfromratandmousesamples.Theantibodywon’trecognizehumanAT2R.

    Anti-AngiotensinIIReceptorType-2(extracellular)-ATTO-488Antibody(#AAR-012-AG)isdirectlylabeledwithanATTO-488fluorescentdye.ATTOdyesarecharacterizedbystrongabsorption(highextinctioncoefficient),highfluorescencequantumyield,andhighphoto-stABIlity.TheATTO-488labelisanalogoustothewellknowndyefluoresceinisothiocyanate(FITC)andcanbeusedwithfilterstypicallyusedtodetectFITC.Anti-AngiotensinIIReceptorType-2(extracellular)-ATTO-488AntibodyhasbeentestedinlivecellimagingandimmunohistochemicalapplicationsandisspeciallysuitedtoexperimentsrequiringsimultaneouslabelingofdifferentMarkers.

    Forresearchpurposesonly,notforhumanuse
    Discovery®抗体是Cambridge Research Biochemicals的目录抗体业务。 CRB是全球第二大最古老的肽公司，也是抗体研究市场的先驱，目前正以Discovery®Antibodies的名义建立一系列新验证的靶标抗体。 Discovery®抗体将专注于研究用抗体（早期药物“发现”），并将补充我们非常成功且长期存在的定制抗体业务（www.crbdiscovery.com）。我们已经以新颖，复杂，稀有和翻译后修饰的特异性抗体而闻名，这些受追捧的靶标将出现在我们的Discovery®Antibody目录范围内。  我们于2017年2月，在发现肽之后一年，并且在詹姆斯·库克船长（他当时最伟大的探险家）于1776年从英国出发，乘坐“ HMS发现”号开始租用新水域和“发现全球各地的新土地。因此，“发现”这个名称似乎适合东北制造商–詹姆斯·库克的故乡。 在1700年代后期对全球新发现土地的追求与医学研究的新“发现”以及2000年人类基因组图谱的发现之间，还有其他相似之处。  有关更多详细信息，请参见有关Discovery®抗体发布的新闻稿。   Cambridge Research Biochemicals  （www.crbdiscovery.com）成立于1980年，是英国领先的定制肽和抗体工具的独立生产商，为全球制药，生命科学和学术领域的研究人员提供产品。其产品的主要应用是定制的实验室规模试剂，用于早期药物发现中的主要研究证据，并支持临床研究中的生物标志物计划。该公司生产各种口味的肽，从简单到修饰，稳定和放射性标记，再到从毫克到克到最高纯度的染料标记。此外，CRB是产生高度特异性和靶向抗体（多克隆抗体和单克隆抗体）的公认专家。荧光染料标记也是重点。自1994年以来，该公司于2016年启动了其首个目录业务DISCOVERY®。

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