Description:

DISCONTINUED–REPLACEMENT:MP0230

Multiplate®COLtestisareagentkit usedforcollageninducedaggregation inresearchof plateletaggregationstudiesfortheevaluationofnormalplateletfunctionontheMultiplate®Analyzer. Sensitivetoacyclooxygenaseinhibition,GpIIb/IIIa antagoNISTsanddeficiencyofGpIIb/IIIareceptors. Fortheresearch ofqualitativeplateletfunctioninhibition.

KitComposition:

Reagents,Composition,StorageandStABIlity

• MP0193:Multiplate®COLtestreagent,Kit,1x1.0mL.50tests. Reagentisstableuntiltheexpirationdateprintedonthe viallabelwhenstoredat2-8°C. Stable7daysafterreconstitution.Donotfreezethereconstitutedreagent.

Alsoavailable

• MP0093: Multiplate®COLtestAliquotVials,Bag, 100 colorcoded(white)microtesttubes.
• MP0130:Multiplate®COLtestreagent,Bag, 1x1.0mL,5colorcoded(white)microtesttubesfor aliquotation.50tests.
• MP0230:Multiplate®COLtestreagent, Kit,3x1.0mL.150tests

MeasurementPrinciple:

TheCOLtestleadstoplateletactivationviatheplateletcollagenreceptor.Forsuitableplateletactivation,thereleaseofendogenousarachidonicacidfromtheplateletphospholipidsisnecessary.ThearachidonicacidisthentransformedtotheplateletagonistthromboxaneA2(TXA2)bytheenzymecyclooxygenase.CyclooxygenaseisblockedbyAspirin®.Therefore,theCOLtestissensitivetoAspirin®action.ThismechanismalsoexplainswhytheCOLtestislessspecifictowardstheactionofAspirin®ascomparedtotheASPItest.IntheASPItest,adefinedamountofarachidonicacidisusedastheactivator.However,inCOLtest,plateletsareactivatedthroughendogenousTXA2andotherTXA2independentmechanisms.

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