This kit enables analysis of apoptosis based on caspase-3/7 activity and its consensus target sequence, Asp-Glu-Val-Asp (DEVD). Caspase-3 and caspase-7 are proteases that are activated during the execution phase of apoptosis. This kit provides a single-step, homogenous assay that is specifically designed for microplate readers. The fluorogenic substrate (Ac-DEVD)₂-R110 contains two DEVD consensus target sequence and is completely hydrolyzed in cell lysate by the enzymes. Hydrolysis of both DEVD peptides releases the green fluorescent dye R110, resulting in a substantial fluorescence increase, with excitation at 490 nm and emission at 520 nm. The assay kit includes the competitive caspase-3/7 inhibitor Ac-DEVD-CHO for use as a negative control. R110 is also provided in the kit for generating a standard curve, which can be used for quantifying caspase-3 activity.  

 

 

Available Configurations:

Configuration	Description	Part Number
Explorer Kit	(1) vial of Rhodamine 110 (80 µM, 1 mL) (2) vials of caspase-3 inhibitor (5 mM, 20 µL) (2) vials of DEVD-R110 (2 mM, 500 µL) (2) vials of lysis buffer (10 mL)Each kit contains enough material for two (2) 96-well or 384-well microplates.	(R8346)

 

 

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