The FLIPR Potassium Assay Kit exploits the permeability of thallium ions (Tl⁺) through both voltage- and ligand-gated potassium (K⁺) channels. In this assay, a novel, highly-sensitive Tl⁺ indicator dye is utilized which produces a bright fluorescent signal upon the binding to Tl⁺ conducted through potassium channels. The intensity of the Tl⁺ signal is proportional to the number of potassium channels in the open state; therefore it provides a functional indication of the potassium channel activities. In addition, one of our proprietary masking dyes is employed to further reduce background fluorescence for improved signal/noise ratio.

• Functional measurement of potassium channel activity in a cell-based assay
• Homogenous no-wash protocol reduces well-to-well variation and simplifies the workflow
• Expanded signal window compared to non-homogenous assay
• Rapid procedure with less hands‐on time

 

 

Available Configurations:

Configuration	Description	Part Number
Explorer Kit	(10) Vials of Component A (1) Bottle of Component B* assay buffer HBSS + Ca2+, Mg2+ and 20 mM HEPES, pH 7.4 (10) Vials of Component C (1) Bottle of 5X chloride-free assay buffer (1) Bottle of potassium sulfate solutin (200 mM) (1) Bottle of thallium sulfate solution (50 mM) Each kit contains enough material for ten (10) 96-well or 384-well microplates. Each vial is sufficient for one (1) microplate experiment.	(R8222)
Bulk Kit	(10) Vials of Component A (10) Vials of Component C (1) Bottle of 5X chloride-free assay buffer (1) Bottle of potassium sulfate solutin (200 mM) (1) Bottle of thallium sulfate solution (50 mM) Each kit contains enough material for one hundred (100) 96-well or 384-well microplates. Each vial is sufficient for ten (10) microplate experiments.	(R8223)
Evaluation Kit	(2) Vials of Component A (1) Bottle of Component B* assay buffer HBSS + Ca2+, Mg2+ and 20 mM HEPES, pH 7.4 (2) Vials of Component C (1) Bottle of 5X chloride-free assay buffer (1) Bottle of potassium sulfate solutin (200 mM) (1) Bottle of thallium sulfate solution (50 mM) Each kit contains enough material for two (2) 96-well or 384-well microplates. Each vial is sufficient for one (1) microplate experiment.	(R8330)

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