5-Bromo-4-chloro-3-indolylβ-D-galactopyranosideismostcommonlyknownas X-Gal.X-galisahistochemicalsubstrateforβ-galactosidasewhichcleavesX-Galtoyieldaninsolubleblueprecipitate.

X-GalisamainstayinmolecularBIOLOGycloningapplicationsinwhichitisusedtodetecttheactivityofβ-galactosidase.X-GalisusedtodetecttheinsertionofforeignDNAintothelacZregionofaplasmidDNA.InsertionofDNAintothelacZregionresultsinthelossofβ-galactosidaseactivity.

X-galisusedtoindicatewhetheracellexpressestheβ-glactosidaseenzyme,whichisencodedbythelacZgene,inatechniquecalledbluewhitescreening.Bacterialcellsthatretainactiveβ-galactosidasewillresultincharacteristicbluecolonies.Successfuldisruptionofthe lacZα genedisruptstheα-complementationoftheβ-galactosidasegeneandtheprecipitatedoesnotform,resultinginwhitecolonies.

 

 

 

AdditionalInformation

AdditionalInformation

Synonyms	BCIG
Product#	X-1015
CAS#	7240-90-6
ChemicalName	5-Bromo-4-Chloro-3-Indolyl-B-D-Galactopyranoside
Formula	C14H15BrClNO6
MW	408.61
Appearance	WhitePowder
Purity	99%(TLC)
Solubility	Soluble2%inDMF
MeltingPoint	236-238°C
BoilingPoint	698.00°Cat760mmHg
StorageTemp	+4°C
Use	Achromogenichistochemicalsubstrateforβ-galactosidaseusedtodetectDNAinsertionsintothelacZregionofplasmidDNA

CHAPS的应用：在实验室中，CHAPS和其他两性离子洗涤剂可用于在分离蛋白之前诱导细胞裂解。它也可以用作二维凝胶电泳过程中的缓冲液，在某些情况下，它比SDS等阴离子洗涤剂更受欢迎。在蛋白质分析之前，可以通过透析从溶液中去除CHAPS。另一种技术利用专门的树脂和旋转柱也可以用来有效地去除皲裂用于监测化学反应或蛋白质结合的紫外/可见光谱学•用于离子交换色谱和等电色谱•用于细胞的膜渗透•以酶特性作为变性缓冲液的成分

MDLNumber	MFCD00005666
ChemACX	X1009969-1
InChI	InChI=1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
SMILES	c1cc(c(c2c1[nH]cc2O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O)Cl)Br