RNasestandsforRibonucleasewhichisaRNAdigestingenzyme.RNaseconsistsof124aminoacidsinonepolypeptidechainwith4disulphidelinkages. Ribonucleasescanbedividedinto Endoribonucleases and Exoribonucleases. RNaseshaveimportantrolesinRNAdegradationandturnoverinallorganisms. RibonucleaseenzymecanunwindtheRNAhelixbycomplexingwithsingle-strandedRNA.RNaseAisanendoribonucleasewithfunctionsinRNAmetabolismandregulationofgeneexpression. Itwasfoundtoplayrolesindiseasessuchasautoimmunediseases,renalinsufficienciesandpancreasdisorder. Morerecently,anantitumoractivitywasalsoreportedforanRNaseAwithcytotoxicandcytostaticproperties. ThemembersofRNaseAsuperfamilyarewidelyexpressedandpresentinserumandtissuesofmammals. Theribonucleolyticactivityinthepancreasofruminantsisparticularlyhigh,perhapstodigestthelargeamountofRNAproducedbystomachmicroorganisms. 

Applications:

TheenzymecanbeusedtohydrolyzeRNAfromproteinsamples.ItiscompatIBLeforuseinRNaseprotectionassays,toremoveunspecificallyboundRNA,intheanalysisofRNAsequences,tohydrolyzeRNAcontainedinproteinsamples,andinthepurificationofDNA.

 

AdditionalInformation

AdditionalInformation

Synonyms	bovinepancreaticribonucleaseA,RNaseAEC3.1.27.5
Product#	R-2000
CAS#	9001-99-4
ChemicalName	RIBONUCLEATE3"-PYRIMIDINOOLIGONUCLEOTIDOHYDROLASE

CHAPS的应用：在实验室中，CHAPS和其他两性离子洗涤剂可用于在分离蛋白之前诱导细胞裂解。它也可以用作二维凝胶电泳过程中的缓冲液，在某些情况下，它比SDS等阴离子洗涤剂更受欢迎。在蛋白质分析之前，可以通过透析从溶液中去除CHAPS。另一种技术利用专门的树脂和旋转柱也可以用来有效地去除皲裂用于监测化学反应或蛋白质结合的紫外/可见光谱学•用于离子交换色谱和等电色谱•用于细胞的膜渗透•以酶特性作为变性缓冲液的成分

MDLNumber	MFCD00132181
ChemACX	X1037961-6